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Team:Technion/8 August 2012
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==Ilya== | ==Ilya== | ||
- Checked the sequencing results for BBa I0462. There is no way it is BBa I0462. A couple of days ago, I noticed that the experience info about this BioBrick states that it is actually a different BioBrick. Trying to clone this part was a waste of time. I will amplify the correct BBa I0462 from BBa F2620 using PCR and clone it downstream to R0062+RS+mCh.<br> | - Checked the sequencing results for BBa I0462. There is no way it is BBa I0462. A couple of days ago, I noticed that the experience info about this BioBrick states that it is actually a different BioBrick. Trying to clone this part was a waste of time. I will amplify the correct BBa I0462 from BBa F2620 using PCR and clone it downstream to R0062+RS+mCh.<br> | ||
| - | - | + | - Glycerol stock of BBa F2620. Freezer position is 3C.<br> |
| + | - Miniprep of BBa F2620. Concentrations are: 1F - 168.5 ng/ul, 2F - 169.5 ng/ul.<br> | ||
| + | - The plate reader results seem to be weird. There is no change with concentration and the auto-fluorescence seems to be very high. Maybe the riboswitch doesn't function as expected. | ||
| + | |||
==Inbal== | ==Inbal== | ||
Revision as of 13:03, 8 August 2012
- Home
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Ilya
- Checked the sequencing results for BBa I0462. There is no way it is BBa I0462. A couple of days ago, I noticed that the experience info about this BioBrick states that it is actually a different BioBrick. Trying to clone this part was a waste of time. I will amplify the correct BBa I0462 from BBa F2620 using PCR and clone it downstream to R0062+RS+mCh.
- Glycerol stock of BBa F2620. Freezer position is 3C.
- Miniprep of BBa F2620. Concentrations are: 1F - 168.5 ng/ul, 2F - 169.5 ng/ul.
- The plate reader results seem to be weird. There is no change with concentration and the auto-fluorescence seems to be very high. Maybe the riboswitch doesn't function as expected.
