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Team:Technion/7 August 2012
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- yea! the bacteria containing the biobrick grew on the LB+Amp plate and today i have taken 5 ml for the starters.<br> | - yea! the bacteria containing the biobrick grew on the LB+Amp plate and today i have taken 5 ml for the starters.<br> | ||
- we decided that we need one more approach for the test plasmids: using xyIE reporter gene from the distribution kit | - we decided that we need one more approach for the test plasmids: using xyIE reporter gene from the distribution kit | ||
| - | so i did transformation to TopTenRB compotent cells and plated 100 and 200 ul on an Amp plate. | + | so i did transformation to TopTenRB compotent cells and plated 100 and 200 ul on an Amp plate. |
==Noa== | ==Noa== | ||
Revision as of 06:41, 8 August 2012
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Ilya
- This morning I came up with a few improvements for our strategy for dividing the phage into workable fragments. I presented them to Hila, who also did some research and we presented everything to Roee. Looks like this idea might actually work.
- We had a group meeting, in which we all presented what we are planing to do.
- I did some preparation for the plate reader experiment tomorrow. Prepared BA and theiphylline stocks. Planned the dilutions and the detailed protocol.
- Put a 5ml starter of pSB1C3+Fus2 for the plate reader tomorrow.
- Put a 10ml starter of pSB1A2+F2620 for minipreps tomorrow.
Inbal
Asaf
Hila
Lior
- yea! the bacteria containing the biobrick grew on the LB+Amp plate and today i have taken 5 ml for the starters.
- we decided that we need one more approach for the test plasmids: using xyIE reporter gene from the distribution kit
so i did transformation to TopTenRB compotent cells and plated 100 and 200 ul on an Amp plate.
