Team:Technion/6 September 2012
From 2012.igem.org
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==Evgeni== | ==Evgeni== | ||
+ | - Transformation result: I had seen growth only on rest plates, few colonies on control plate. I've put plates in 4C | ||
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+ | - Centrifugation and freezing of pPROLar starters | ||
==Shahar== | ==Shahar== |
Revision as of 07:24, 11 September 2012
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Ilya
Inbal
Asaf
Today I tried the fusion PCR of the RiboSwitch with the K1f polymerase gene with a lower gradient temperture (55,56,57C).
I did it for greater resulution.
I ran the PCR pruducts on agarose gel to test them. I got good results. I can see clearly the 3.2kb bands, the other
bands are for the K1F polymerase (2.7kb) and for the RiboSwitch (500bp).
Because I found the right Tm, I used Tm=57C for the fusion PCR of the Riboswitch with the rest of the polymerases genes.
Hila
Lior
Noa
Evgeni
- Transformation result: I had seen growth only on rest plates, few colonies on control plate. I've put plates in 4C
- Centrifugation and freezing of pPROLar starters