Team:Technion/30 August 2012
From 2012.igem.org
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Ilya
Inbal
Asaf
I tried again the PCR of the 3 polymerase genes that failed the previues PCR (T7*,N4,K1F), now with Tm of 66C.
I tried this Tm because I got results with this Tm before (29.8 polymerase gel 2). This time I got it right,
I got the 2.7 kb bands I was expecting (althouge N4 is rather week).
image:30.8 polymerase test gel4-view.tif