Team:Technion/30 August 2012
From 2012.igem.org
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I tried this Tm because I got results with this Tm before (29.8 polymerase gel 2). This time I got it right,<br> | I tried this Tm because I got results with this Tm before (29.8 polymerase gel 2). This time I got it right,<br> | ||
I got the 2.7 kb bands I was expecting (althouge N4 is rather week). | I got the 2.7 kb bands I was expecting (althouge N4 is rather week). | ||
- | [[image:30.8 polymerase test gel4-view. | + | [[image:30.8 polymerase test gel4-view.tiff]] |
==Hila== | ==Hila== | ||
Revision as of 14:25, 4 September 2012
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Ilya
Inbal
Asaf
I tried again the PCR of the 3 polymerase genes that failed the previues PCR (T7*,N4,K1F), now with Tm of 66C.
I tried this Tm because I got results with this Tm before (29.8 polymerase gel 2). This time I got it right,
I got the 2.7 kb bands I was expecting (althouge N4 is rather week).
File:30.8 polymerase test gel4-view.tiff
Hila
Lior
Noa
Evgeni
- Transformation of TOP10 RB cells with pPROLar+Bba_015 ligated part
- Plating of transformed cells on Kanamycin plates - 100 ul and rest
Shahar & Rachel
We tried to do the failed PCR's from yesterday, but with lower temprature. For N4 (with A2 & plac/ara primers)we tried with temprature gradient.