Team:Technion/26 July 2012
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- Cleaned after CIP. Concentrations: R0062 SpeI PstI CIP - 9.6 ng/μl, J23119 SpeI PstI CIP - 22.5 ng/μl, pSB1C3 EcoRI PstI CIP - 15.6 ng/μl.<br> | - Cleaned after CIP. Concentrations: R0062 SpeI PstI CIP - 9.6 ng/μl, J23119 SpeI PstI CIP - 22.5 ng/μl, pSB1C3 EcoRI PstI CIP - 15.6 ng/μl.<br> | ||
- Ligations: R0062+Fus1, J23119+Fus1, R0062 control, J23119 control, pUC19 positive control.<br> | - Ligations: R0062+Fus1, J23119+Fus1, R0062 control, J23119 control, pUC19 positive control.<br> | ||
- | - Transformations of all the ligations. They are in the 37 incubator overnight. | + | - Transformations of all the ligations. They are in the 37 incubator overnight.<br> |
+ | - Prepared a gel for colony PCR. | ||
==Inbal== | ==Inbal== |
Latest revision as of 11:56, 26 July 2012
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Ilya
- Cleaned after CIP. Concentrations: R0062 SpeI PstI CIP - 9.6 ng/μl, J23119 SpeI PstI CIP - 22.5 ng/μl, pSB1C3 EcoRI PstI CIP - 15.6 ng/μl.
- Ligations: R0062+Fus1, J23119+Fus1, R0062 control, J23119 control, pUC19 positive control.
- Transformations of all the ligations. They are in the 37 incubator overnight.
- Prepared a gel for colony PCR.