From 2012.igem.org
Team:Technion/23_July_2012
Ilya
- The ligations and transformations worked. The yield was very small but we still got a few colonies on the plates that were plated with the rest of the cells (after centrifugation).
- Did colony PCR reactions on 3 colonies from Fus1 HF, 3 colonies from Fus1 GC and 6 colonies of Fus2.
- Only 1 colony of Fus1 gave positive results (#1) and 4 colonies of Fus2 gave positive results (#7, #9, #10, #11).
- Put starters 10 ml each of the only positive colony of Fus1 and two positive colonies of Fus2 (#7 and #11). I also left the plate with the isolated colonies in the 37 incubator to grow some more. Hopefully, tomorrow we can miniprep the plasmids and send them for sequencing.
Inbal
Asaf
Hila
Lior
Noa
Evgeni
Shahar
Rachel
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