From 2012.igem.org
Team:Technion/22_July_2012
Ilya
- Sterilized ependorfs.
- Transformed the ligation products (Fus1 HF, Fus1 GC, Fus 2, ligation control). Plated on plates with Chloramphenicol antibiotics. In addition Transformed pUC19 with Amp resistance as a positive control and plated on plates with Amp. For Each sample, 100 ul were plated and the rest was centrifuged at 4000g for 5 min and then plated. Incubated in 37 degrees overnight.
- Took the readymix and DNTPs from Sharons lab and put in our -30 freezer.
- Prepared two 1% agarose gels for colony PCR. Use the small combs next time.
Inbal
Asaf
Hila
Lior
Noa
Evgeni
Shahar
Rachel
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