Team:Technion/20 September 2012
From 2012.igem.org
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+ | 4 miniprep for each RNAP(K1F, T3, N4)- at total 12 miniprep. | ||
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Revision as of 12:11, 26 September 2012
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Ilya
Wiki, wiki, wiki...
Inbal
- The transformation worked! I got colonies on both transformation!(of T3 and also In1 with xyIE/ALP)- so I made starters for them.. The plates were stored at 4C.
Asaf
I transformed my lygated plasmid+inserts to Top 10 bacteria strain.
Hila
- Overnight ligation to the restriction products, into pSB1C3+MCS.
- PCR for all fragments with 100bp fragments overlap for the Gibson assembly tryout…
- Ladder experiment's for the BEST LADDER EVERRRR!!!
Lior
Preparing the shipping plasmids
Noa
Preparing the shipping plasmids
Evgeni
Shahar
Rachel
4 miniprep for each RNAP(K1F, T3, N4)- at total 12 miniprep.