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Team:Technion/19 August 2012
From 2012.igem.org
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==Ilya== | ==Ilya== | ||
| + | - All the white colony PCR colonies were positive on the gel. I picked one from each clone and put a starter. | ||
==Inbal== | ==Inbal== | ||
| - | + | Today was pretty theoretical for me- I read and planned the Gibson assembly, Also I found out that it isn't piece of cake. <br> | |
| + | In addition, I measured the concentration of the mini preped pSB1C3+SP6 gene product: 105ng/ul. | ||
==Asaf== | ==Asaf== | ||
| Line 14: | Line 16: | ||
To do list: <br> | To do list: <br> | ||
| - | 1. PCR for Chris's plasmids: <br> | + | 1. PCR for Chris's plasmids: promoter plasmids (5 total) <br> |
| - | 2. PCR purification for PCR products of xyIE. <br> | + | |
| + | 2. a. PCR purification for PCR products of xyIE. <br> | ||
| + | 2. b. Plates for plating the ligated products- with XXX antibiotic. <br> | ||
| + | |||
3. PCR purification for PCR products of Chris's plasmids. <br> | 3. PCR purification for PCR products of Chris's plasmids. <br> | ||
| + | |||
| + | 4. Restriction with XmaI + XhoI for: <br> | ||
| + | - xyIE PCR products <br> | ||
| + | - Chris's promoter plasmids (5 reactions total) <br> | ||
| + | |||
| + | 5. PCR purification for cut products of xyIE and Chris's plasmids <br> | ||
| + | |||
| + | 6. Ligation of xyIE and Chris's plasmids: 5 reactions total. <br> | ||
| + | |||
If there is time: PCR for alkaline phosphatase and running on Gel. | If there is time: PCR for alkaline phosphatase and running on Gel. | ||
==Evgeni== | ==Evgeni== | ||
Latest revision as of 21:00, 22 August 2012
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Ilya
- All the white colony PCR colonies were positive on the gel. I picked one from each clone and put a starter.
Inbal
Today was pretty theoretical for me- I read and planned the Gibson assembly, Also I found out that it isn't piece of cake.
In addition, I measured the concentration of the mini preped pSB1C3+SP6 gene product: 105ng/ul.
Asaf
Hila
Lior
Noa
To do list:
1. PCR for Chris's plasmids: promoter plasmids (5 total)
2. a. PCR purification for PCR products of xyIE.
2. b. Plates for plating the ligated products- with XXX antibiotic.
3. PCR purification for PCR products of Chris's plasmids.
4. Restriction with XmaI + XhoI for:
- xyIE PCR products
- Chris's promoter plasmids (5 reactions total)
5. PCR purification for cut products of xyIE and Chris's plasmids
6. Ligation of xyIE and Chris's plasmids: 5 reactions total.
If there is time: PCR for alkaline phosphatase and running on Gel.
