Team:Technion/19 August 2012
From 2012.igem.org
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1. PCR purification for PCR products of xyIE. <br> | 1. PCR purification for PCR products of xyIE. <br> | ||
2. Restriction with XmaI + XhoI for: <br> | 2. Restriction with XmaI + XhoI for: <br> | ||
- | + | - xyIE PCR products <br> | |
- | + | - of Chris's promoter plasmids <br> | |
3. PCR purification for cut products of xyIE and Chris's plasmids <br> | 3. PCR purification for cut products of xyIE and Chris's plasmids <br> | ||
4. Ligation of xyIE and Chris's plasmids: 5 reactions total. <br> <br> | 4. Ligation of xyIE and Chris's plasmids: 5 reactions total. <br> <br> |
Revision as of 09:47, 18 August 2012
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Ilya
Inbal
Asaf
Hila
Lior
Noa
1. PCR purification for PCR products of xyIE.
2. Restriction with XmaI + XhoI for:
- xyIE PCR products
- of Chris's promoter plasmids
3. PCR purification for cut products of xyIE and Chris's plasmids
4. Ligation of xyIE and Chris's plasmids: 5 reactions total.
5. If there is time: PCR for alkaline phosphatase and running on Gel.