From 2012.igem.org
Team:Technion/13_September_2012
Ilya
- Began working on adding our parts to the registry.
Inbal
Asaf
I figured that I don't have enough insert (Riboswitch with polymerase) for ligation with the Psb1c3.
I have decided to extract more insert from gel from the product I got from the fusion PCR with Tm of 55C.
I couldn't find the T7* product. I have already used both products of T7.
So I ran the Riboswitch with the K1F, T3 and N4 polymerase genes on an extraction gel.
I got the expected bands,but I didn't get the 1Kb pattern, and cut the 3.2 kb bands (as I expected from previuos gels).
I cleaned the DNA from the gel.
I cut all of my inserts (Riboswitch with different polymerases) with EcoRI and PstI.
I also helped Evgeni and made glycerol stocks of his starters, and also centerfuge the starters so that he could
miniprep them tomorrow.
Hila
Lior
Noa
Evgeni
Shahar
Rachel
|
"
