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Team:Technion/13 September 2012
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==Asaf== | ==Asaf== | ||
| - | + | I figured that I don't have enough insert (Riboswitch with polymerase) for ligation with the Psb1c3.<br> | |
| + | I have decided to extract more insert from gel from the product I got from the fusion PCR with Tm of 55C.<br> | ||
| + | I couldn't find the T7* product. I have already used both products of T7.<br> | ||
| + | So I ran the Riboswitch with the K1F, T3 and N4 polymerase genes on an extraction gel.<br> I got the expected bands,but I didn't get the 1Kb pattern, and cut the 3.2 kb bands (as I expected from previuos gels).<br> | ||
| + | <gallery perrow="4" widths=150px heights=150px style="color: black; border: 5px ridge #A7A3BF; font: 13px david; background: azure"> | ||
| + | Image:13.9_fusion_RS+poly_grad_extraction_gel2-view.jpg | ||
| + | </gallery> | ||
| + | I cleaned the DNA from the gel.<br> | ||
| + | I cut all of my inserts (Riboswitch with different polymerases) with EcoRI and PstI. | ||
==Hila== | ==Hila== | ||
Revision as of 12:34, 15 September 2012
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Ilya
- Began working on adding our parts to the registry.
Inbal
Asaf
I figured that I don't have enough insert (Riboswitch with polymerase) for ligation with the Psb1c3.
I have decided to extract more insert from gel from the product I got from the fusion PCR with Tm of 55C.
I couldn't find the T7* product. I have already used both products of T7.
So I ran the Riboswitch with the K1F, T3 and N4 polymerase genes on an extraction gel.
I got the expected bands,but I didn't get the 1Kb pattern, and cut the 3.2 kb bands (as I expected from previuos gels).
 |
I cleaned the DNA from the gel.
I cut all of my inserts (Riboswitch with different polymerases) with EcoRI and PstI.
