From 2012.igem.org
Team:Technion/13_August_2012
Ilya
Inbal
-Transformation of the ligated products (pSB1C3+SP6) to 100ul component cells (TOP10 Rb bacteria).
-Recovery on SOB for 1 hr ai 37C in the shaker.
-Plating 100ul and 900ul on LB+CM plates.
-I stores the plates in 37C incubator overnight.
Asaf
Hila
Lior
Noa
PCR for xyIE and Alkaline Phosphatase, gel electroporation and PCR purification.
Starters for Chris's plasmids:
5 microL spectinomycin + 5 ml LB X 6 ???
13 microL chloromphenicol + 5 ml LB X 6 ???
Evgeni
Shahar
Rachel
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