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Team:Technion/12 September 2012
From 2012.igem.org
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==Asaf== | ==Asaf== | ||
| - | I need to insert the long Riboswitch+polymerase genes to Psb1c3 plasmid.I minipreped the strain that contain the Psb1c3 with the MCS.<br> | + | I need to insert the long Riboswitch+polymerase genes to Psb1c3 plasmid.I minipreped the strain that contain the Psb1c3 with the MCS.<br> I got good DNA concentrations (75.5-120.5 ng/ul).<br> |
I cut the Psb1c3 with the MCS with EcoRI and PstI restriction enzymes.<br> | I cut the Psb1c3 with the MCS with EcoRI and PstI restriction enzymes.<br> | ||
I ran the cut Psb1c3 on gel for extraction. I got the expected 2kb bands.<br> | I ran the cut Psb1c3 on gel for extraction. I got the expected 2kb bands.<br> | ||
Revision as of 12:12, 15 September 2012
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Ilya
- Repeated plate reader for tac+RS+mCherry and tac+RBS+mCherry in pCP. Got similar results to previous experiments. Constitutive expression of mCherry, without any effect of theophylline concentrations. Moreover, the tac+RBS+mCherry gave lower readings than tac+RS+mCherry, in contrast to expectations and one previous experiment. After talking to Roee, we decided to summarize the results along with some calculations and assumptions for the failure of the riboswitch. We will still submit this part to the registry, along with all of our results.
- Prepared a 0.4% gel for the gibson attempts on the phage. Unfortunately, we didn't have time to do the experiments today.
- Began setting up the restrictions for characterizing the MCS plasmid. Shahar and Hila will finish the digestions and run the results on a gel.
Inbal
Asaf
I need to insert the long Riboswitch+polymerase genes to Psb1c3 plasmid.I minipreped the strain that contain the Psb1c3 with the MCS.
I got good DNA concentrations (75.5-120.5 ng/ul).
I cut the Psb1c3 with the MCS with EcoRI and PstI restriction enzymes.
I ran the cut Psb1c3 on gel for extraction. I got the expected 2kb bands.
 |
Shahar cleaned the DNA from the gel I extracted.
