Team:TU Munich/Results/Biobricks
From 2012.igem.org
VolkerMorath (Talk | contribs) |
VolkerMorath (Talk | contribs) |
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|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801037 BBa_K801037] || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801010 BBa_K801010] || ||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801076 BBa_K801076] || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801096 BBa_K801096] || || | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801037 BBa_K801037] || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801010 BBa_K801010] || ||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801076 BBa_K801076] || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801096 BBa_K801096] || || | ||
|- | |- | ||
- | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801038 BBa_K801038] || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801001 BBa_K801011] || ||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801077 BBa_K801077] || | + | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801038 BBa_K801038] || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801001 BBa_K801011] || ||[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801077 BBa_K801077] || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801097 BBa_K801097] || || |
|- | |- | ||
|[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801039 BBa_K801039] || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801001 BBa_K801011] || || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801098 BBa_K801098] || || | |[http://partsregistry.org/wiki/index.php?title=Part:BBa_K801039 BBa_K801039] || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801001 BBa_K801011] || || || [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801098 BBa_K801098] || || | ||
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[http://partsregistry.org/Part:BBa_K268000:Experience#User_Reviews| Forbidden restriction sites in pSB6A0 (BBa_K268000g)] | [http://partsregistry.org/Part:BBa_K268000:Experience#User_Reviews| Forbidden restriction sites in pSB6A0 (BBa_K268000g)] | ||
+ | |||
+ | ==Improvement of an existing BioBrick: <partinfo><BBa K801100</partinfo>== | ||
+ | In 2012 the [http://partsregistry.org Registry of Standard Biological Parts] defined <partinfo>BBa_J04450</partinfo> as the standard shipping part that is required for submission of backbones, creating the need for a RFC25 compatible standard shipping part.<br> | ||
+ | |||
+ | We extended the standard compability of the RFP coding device (<partinfo>BBa_J04450</partinfo>) to RFC10 and [http://hdl.handle.net/1721.1/45140 RFC25] by adding the NgoMIV and AgeI restriction sites into the prefix and suffix of this part. Additionally two AgeI restriction sites that were present in the generator itself were deleted. <br><br> | ||
+ | |||
+ | This part may be used as a standard insert for RFC10 as well as [http://hdl.handle.net/1721.1/45140 RFC25] backbones. This improvement became necessary because insertion of <partinfo>BBa_J04450</partinfo> into a RFC25 compatible backbone leads to the deletion of the desired [http://hdl.handle.net/1721.1/45140 RFC25] restriction sites that are needed for protein fusions. |
Revision as of 12:26, 22 October 2012
Contents |
BioBricks
BioBricks constructed during iGEM 2012
light inducible (A) | ethanol inducible (B) | constitutive (C) | limonene (D) | caffeine (E) | xanthohumol (F) | thaumatin (H) | ||
BBa_K801031 | BBa_K801020 | BBa_K801000 | BBa_K801061 | BBa_K801070 | BBa_K801090 | BBa_K801080 | ||
BBa_K801032 | BBa_K801001 | BBa_K801062 | BBa_K801071 | BBa_K801091 | BBa_K801081 | |||
BBa_K801033 | BBa_K801002 | BBa_K801063 | BBa_K801072 | BBa_K801092 | ||||
BBa_K801034 | BBa_K801003 | BBa_K801064 | BBa_K801073 | BBa_K801093 | ||||
BBa_K801035 | BBa_K801004 | BBa_K801065 | BBa_K801074 | BBa_K801094 | ||||
BBa_K801036 | BBa_K801010 | BBa_K801066 | BBa_K801075 | BBa_K801095 | ||||
BBa_K801037 | BBa_K801010 | BBa_K801076 | BBa_K801096 | |||||
BBa_K801038 | BBa_K801011 | BBa_K801077 | BBa_K801097 | |||||
BBa_K801039 | BBa_K801011 | BBa_K801098 | ||||||
BBa_K801040 | BBa_K801012 | |||||||
BBa_K801041 | ||||||||
BBa_K801043 |
List of all Parts
Experiences with existing BioBricks
Rating working BioBricks
Yeast Integration Vector BBa_K300007 (Partly showed functionality)
Identification of incorrect BioBricks
Forbidden restriction sites in pSB6A0 (BBa_K268000g)
Improvement of an existing BioBrick: <partinfo><BBa K801100</partinfo>
In 2012 the Registry of Standard Biological Parts defined <partinfo>BBa_J04450</partinfo> as the standard shipping part that is required for submission of backbones, creating the need for a RFC25 compatible standard shipping part.
We extended the standard compability of the RFP coding device (<partinfo>BBa_J04450</partinfo>) to RFC10 and RFC25 by adding the NgoMIV and AgeI restriction sites into the prefix and suffix of this part. Additionally two AgeI restriction sites that were present in the generator itself were deleted.
This part may be used as a standard insert for RFC10 as well as RFC25 backbones. This improvement became necessary because insertion of <partinfo>BBa_J04450</partinfo> into a RFC25 compatible backbone leads to the deletion of the desired RFC25 restriction sites that are needed for protein fusions.