Team:TU Munich/Modeling/Gal1 Promoter

From 2012.igem.org

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=== Parameters ===
=== Parameters ===
 +
{|cellspacing="0" border="1"
 +
|'''Name'''
 +
|'''Description'''
 +
|'''Prior?'''
 +
|-
 +
| μ
 +
| Scaling factor
 +
| NO
 +
|-
 +
| k<sub>i</sub>
 +
| Induced transcription rate
 +
| YES
 +
|-
 +
| &alpha;
 +
| Leaky transcription rate
 +
| NO
 +
|-
 +
| &gamma;<sub>1</sub>
 +
| mRNA degradation rate
 +
| NO
 +
|-
 +
| k<sub>2</sub>
 +
| Protein synthesis rate
 +
| NO
 +
|-
 +
| &gamma<sub>2</sub>
 +
| Protein degradation rate
 +
| NO
 +
|}
=== Data ===
=== Data ===

Revision as of 14:52, 11 September 2012

Contents

Gal1 Promoter

Model Specification

TUM12 Galpromeq.jpg

Model Equations

We used a two species approach for mRNA and Protein concentrations. x1 and x2 represent the mRNA and protein levels for the induced promoter and x3 and x4 the respective levels for the uninduced promoter.

As no analysis was done with several galactose concentrations hence the hill function that normally models the response to the concentration was replaced by a single factor to improve identifiability.

Parameters

Name Description Prior?
μ Scaling factor NO
ki Induced transcription rate YES
α Leaky transcription rate NO
γ1 mRNA degradation rate NO
k2 Protein synthesis rate NO
&gamma2 Protein degradation rate NO

Data

Profile Likelihood

Markov Analysis

Joint Distribution

Credibility Intervals

Sensitivity Analysis


Reference

Retrieved from "http://2012.igem.org/Team:TU_Munich/Modeling/Gal1_Promoter"