Team:Shenzhen/Project/YAO.Genome
From 2012.igem.org
(Difference between revisions)
Line 25: | Line 25: | ||
<h5>Introduction</h5> | <h5>Introduction</h5> | ||
<ul> | <ul> | ||
- | <p>YAO.Genome is the core part of YAO which’s similar to genomes of mitochondria and chloroplasts but ready to be embedded any new system. Here, we proposed YAO.Genome has two features, self-replication and high capacity of genes. Construction of YAO.Genome contains three stages. First, transform a GFP gene into the mitochondria by homologous recombination. Second, build shuttle plasmid which can replicate both in <i>E. coli</i> and mitochondria, but only contain a GFP gene. And then we introduce it into MT. Third, redesign a genome satisfying all the criteria as we mentioned before. | + | <p>YAO.Genome is the core part of YAO which’s similar to genomes of mitochondria and chloroplasts but ready to be embedded any new system. Here, we proposed YAO.Genome has two features, self-replication and high capacity of genes. Construction of YAO.Genome contains three stages. First, transform a GFP gene into the mitochondria by homologous recombination. Second, build shuttle plasmid which can replicate both in <i>E. coli</i> and mitochondria, but only contain a GFP gene. And then we introduce it into MT. Third, redesign a genome satisfying all the criteria as we mentioned before. We fulfilled the first and second parts, in both experiments mitochondria expressed GFP, and drew picture of our imagination of the last part.</p> |
</ul> | </ul> | ||
</div> | </div> |
Revision as of 09:20, 26 September 2012