Team:Shenzhen/Project/YAO.Genome
From 2012.igem.org
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<ul> | <ul> | ||
<li>Homologous Recombination</li> | <li>Homologous Recombination</li> | ||
- | <p>First, transform a GFP gene into the mitochondria by homologous recombination.</p> | + | <p>First, transform a GFP gene into the mitochondria by homologous recombination.</p></ul> |
- | As we all know, tungsten powder is bigger than recombinant plasmid. While covering tungsten powder with recombinant plasmid and 和重组质粒一块打到细胞里面并且可以让成功打进去的细胞在筛选培养基存活的那个质粒, both of these plasmids will be appear on tungsten powder. | + | <ul><p>As we all know, tungsten powder is bigger than recombinant plasmid. While covering tungsten powder with recombinant plasmid and 和重组质粒一块打到细胞里面并且可以让成功打进去的细胞在筛选培养基存活的那个质粒, both of these plasmids will be appear on tungsten powder.</p></ul> |
- | After using gene gun to inject plasmids into Rho 0 cell, 用来筛选的那个培养基的名字或者功能加名字 will be used to select cells with plasmids in them, which are called Rho- cells. After Rho- cell mated with Rho+ cell, homologous recombination will happen. Mitochondria in them will fuse together, and a cell with GFP gene in it will be obtained if luckily. | + | <ul><p>After using gene gun to inject plasmids into Rho 0 cell, 用来筛选的那个培养基的名字或者功能加名字 will be used to select cells with plasmids in them, which are called Rho- cells.</p></ul> |
+ | <ul><p>After Rho- cell mated with Rho+ cell, homologous recombination will happen. Mitochondria in them will fuse together, and a cell with GFP gene in it will be obtained if luckily.</p> | ||
<li>Shuttle Plasmid</li> | <li>Shuttle Plasmid</li> |
Revision as of 01:36, 25 September 2012