Team:Shenzhen/Project/YAO.Channel
From 2012.igem.org
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- | To achieve the goal that various proteins can be equipped with the proper signal peptides so as to be assembled onto membranes of mitochondria or be targeted into the matrix, we first standardize the BioBricks for signal peptides. By referring to dozens of documents, we picked out 3 kinds of signal peptides for mitochondrion-targeting proteins:</p> | + | To achieve the goal that various proteins can be equipped with the proper signal peptides so as to be assembled onto membranes of mitochondria or be targeted into the matrix, we first standardize the BioBricks for signal peptides. By referring to dozens of documents, we picked out 3 kinds of signal peptides for mitochondrion-targeting proteins:</p></ul> |
- | <p>1) directing proteins onto outer membrane, which can be used to help assemble the Toc proteins onto mitochondria outer membrane;</p> | + | <ul><p>1) directing proteins onto outer membrane, which can be used to help assemble the Toc proteins onto mitochondria outer membrane;</p></ul> |
- | <p>2) directing proteins onto inner membrane, which can be used to help assemble the Tic (Translocase of the Inner membrane of Chloroplast)proteins onto mitochondria inner membrane;</p> | + | <ul><p>2) directing proteins onto inner membrane, which can be used to help assemble the Tic (Translocase of the Inner membrane of Chloroplast)proteins onto mitochondria inner membrane;</p></ul> |
- | <p>3) directing proteins into matrix, which can be used to guide the enzymes or transcriptional factors into matrix of YAO.</p></ul> | + | <ul><p>3) directing proteins into matrix, which can be used to guide the enzymes or transcriptional factors into matrix of YAO.</p></ul> |
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<h5>A New Transport Machinery</h5> | <h5>A New Transport Machinery</h5> | ||
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When the Toc and Tic proteins were successfully assembled onto the membranes of mitochondria, basically speaking the goal for building up a new transport machinery is achieved. The Toc and Tic proteins will act in tandem to transport proteins across the two membranes of YAO into the matrix, as long as the proteins to be transported are equipped with the signal peptides that can be recognized by Toc34 (the Toc34-targeting signal peptides BioBricks is not available presently).</p></ul> | When the Toc and Tic proteins were successfully assembled onto the membranes of mitochondria, basically speaking the goal for building up a new transport machinery is achieved. The Toc and Tic proteins will act in tandem to transport proteins across the two membranes of YAO into the matrix, as long as the proteins to be transported are equipped with the signal peptides that can be recognized by Toc34 (the Toc34-targeting signal peptides BioBricks is not available presently).</p></ul> | ||
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- | <p>Moreover, to endue YAO with independency in protein transit, i.e. all the proteins for YAO should be transported via only YAO.channel rather than the original one—TOM complex, we will try to knock down all Tom proteins, and finally select the yeast whose transport machinery is YAO.channel.</p></ul> | + | <p> Moreover, to endue YAO with independency in protein transit, i.e. all the proteins for YAO should be transported via only YAO.channel rather than the original one—TOM complex, we will try to knock down all Tom proteins, and finally select the yeast whose transport machinery is YAO.channel.</p></ul> |
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Revision as of 07:11, 25 September 2012