Team:SUSTC-Shenzhen-B/achievements

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{{tempalte:sustc_shenzhen_b/1}}
 
<html>
<html>
<head>
<head>
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<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
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<meta charset="utf-8" />
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<title>SUSTC iGEM Theme - Free CSS Template</title>
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<meta name="description" content="" />
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<meta name="keywords" content="beauty class, free theme, website design, bokehs, pink, orange, templatemo, CSS, HTML" />
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<meta name="author" content="tengattack" />
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<meta name="description" content="Beauty Class Theme, pinky background gradient with bokehs, free CSS template provided by templatemo.com" />
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<meta http-equiv="Content-Type" content="text/html; charset=utf-8" />
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<title>Title</title>
<style type="text/css">
<style type="text/css">
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<!--
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#globalWrapper {width: 100%;  }
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.STYLE13 {color: #0000FF}
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#top-section {width: 100%; height:100%; border:none;}
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-->
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#p-logo {display:none;}
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#search-controls {display:none;}
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.printfooter {display:none;}
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#footer-box {border:none;}
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.firstHeading {display:none;}
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#content { border:none !important; width:1024px !important; background: url('') !important;}
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#bodyContent {border:none;  }
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#catlinks {display:none; }
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#footer-box {display:none; }
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#menubar {display:none; }
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/* googleapis-font */
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@font-face {
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  font-family: 'Open Sans';
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  font-style: normal;
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  font-weight: 700;
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  src: local('Open Sans Bold'), local('OpenSans-Bold'), url(http://themes.googleusercontent.com/static/fonts/opensans/v6/k3k702ZOKiLJc3WVjuplzHhCUOGz7vYGh680lGh-uXM.woff) format('woff');
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}
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@font-face {
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  font-family: 'Open Sans';
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  font-style: normal;
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  font-weight: 600;
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  src: local('Open Sans Semibold'), local('OpenSans-Semibold'), url(http://themes.googleusercontent.com/static/fonts/opensans/v6/MTP_ySUJH_bn48VBG8sNSnhCUOGz7vYGh680lGh-uXM.woff) format('woff');
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}
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@font-face {
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  font-family: 'Open Sans';
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  font-style: normal;
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  font-weight: 400;
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  src: local('Open Sans'), local('OpenSans'), url(http://themes.googleusercontent.com/static/fonts/opensans/v6/cJZKeOuBrn4kERxqtaUH3T8E0i7KZn-EPnyo3HZu7kw.woff) format('woff');
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}
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/* inner */
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.pagenavi{clear:both;padding:0}.pagenavi a,.pagenavi a:visited{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#727272}.pagenavi a:hover{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#b03121;text-decoration:none}.pagenavi .current{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px;color:#b03121}.pagenavi .pages{background:#dfddc7;display:inline-block;margin:0 8px 0 0;padding:3px 10px}#breadcrumb{text-align:right;margin-bottom:34px;clear:right}.post{margin-bottom:30px;padding-bottom:30px;clear:both;border-bottom:1px solid #dfddc7}.post img{padding:5px;background:#d2d0ba;width:590px;height:236px}.single{padding-bottom:20px}.postimg{margin-bottom:20px}.posttitle{margin:0 0 5px 0}.posttitle,.posttitle a{font-size:18px;color:#3f4432}.posttitle a:hover{text-decoration:none}.entry-text{overflow:hidden;padding-left:30px}.entry-text h2{padding-top:5px}.entry-content{margin:0;padding:12px 0 5px 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textarea{width:95%;float:left;font-style:italic;color:#727272;font-size:11px;font-family:Arial,Helvetica,sans-serif}#message{margin-left:0;font-weight:700;color:red}#message h2{}#message p{margin:6px 0}.note{color:#d45454}#contactform .button{cursor:pointer;margin-top:20px;clear:both;float:left}
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 +
/* style */
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html,body{height:100%}body{font-family:Arial,Helvetica,sans-serif;font-size:12px;color:#727272;margin:0;padding:0;line-height:20px;background:#d6d4c0 url(/wiki/images/d/d7/Sustc-b1-pattern.png) repeat}*{margin:0;padding:0}:focus{outline:0}form{margin:0;padding:0}hr{border-width:0;height:1px;line-height:0;margin:30px 0;page-break-after:always;text-align:center;width:100%;clear:both;color:#dfddc7;background-color:#dfddc7}.clearfix:before,.clearfix:after{content:'\0020';display:block;overflow:hidden;visibility:hidden;width:0;height:0}.clearfix:after{clear:both}.clearfix{zoom:1}.clear,.clr{clear:both;display:block;overflow:hidden;visibility:hidden;width:0;height:0}h1,h2{margin-bottom:25px}h3,h4,h5,h6{margin-bottom:10px}h1{font-size:26px}h2{font-size:20px}h3{font-size:16px}h4{font-size:14px}h5{font-size:12px}h6{font-size:10px}h1,h2{font-weight:400;font-family:'Open Sans',sans-serif;color:#404631}h3,h4,h5,h6{font-weight:600;font-family:'Open 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li{background:url("/wiki/images/6/6a/Sustc-b1-square.gif") no-repeat 0 14px;line-height:25px;list-style-type:none;margin:0;padding:2px 0 2px 10px;border-bottom:1px solid #dfddc7}.bullet.arrow li{background:url("/wiki/images/b/b4/Sustc-b1-arrow.gif") no-repeat 0 9px;line-height:25px;list-style-type:none;margin:0;padding:0 0 0 10px;border-bottom:0}.bullet.arrow2 li{background:url("/wiki/images/d/d8/Sustc-b1-arrow.png") no-repeat 0 10px;line-height:25px;list-style-type:none;margin:0;padding:0 0 0 18px;border-bottom:0}.tabcontainer{margin:0 0 20px 0}ul.tabs{margin:0 0 -3px 0;padding:0 0 2px 0;list-style:none;height:35px;width:100%;border-bottom:1px solid #e1dfc9}ul.tabs li{float:left;margin:0 1px 0 0;padding:0 0;line-height:35px;height:35px;position:relative}ul.tabs li:last-child{background:transparent}ul.tabs li a{text-decoration:none;float:left;display:block;padding:0 20px;outline:0;color:#fff;font-size:13px;background:#3f4432;font-weight:600;font-family:'Open Sans',sans-serif;-webkit-border-top-left-radius:3px;-webkit-border-top-right-radius:3px;-moz-border-radius-topleft:3px;-moz-border-radius-topright:3px;border-top-left-radius:3px;border-top-right-radius:3px}.tab-content{padding:20px 0}ul.tabs li:hover{}ul.tabs li.active{}html ul.tabs li.active a{color:#b03121;background:#e1dfc9;-webkit-border-top-left-radius:3px;-webkit-border-top-right-radius:3px;-moz-border-radius-topleft:3px;-moz-border-radius-topright:3px;border-top-left-radius:3px;border-top-right-radius:3px}#tab-body{padding:0 20px;background:#e1dfc9;border:0;-webkit-border-radius:3px;-webkit-border-top-left-radius:0;-moz-border-radius:3px;-moz-border-radius-topleft:0;border-radius:3px;border-top-left-radius:0}#toggle{margin:0 0 20px 0}h2.trigger{padding:0 0;margin:0;font-size:13px;background:transparent;font-family:arial;color:#3f4432}h2.trigger span{text-decoration:none;display:block;background:url(/wiki/images/9/93/Sustc-b1-toggle.png) no-repeat 0 5px;padding-left:35px;cursor:pointer;line-height:35px}h2.active span{background:url(/wiki/images/c/cd/Sustc-b1-toggle-down.png) no-repeat 0 5px}h2.trigger a:hover{color:#efeed9}h2.active{background:transparent;color:#af3728}.toggle_container{margin:0 0 1px 0;padding:5px 15px;overflow:hidden;clear:both;background:transparent}.toggle_container .block{padding:0 0 0 20px}.toggle_container .block p{padding-bottom:10px;margin:0}#sidebar{width:300px;float:left;padding:0 0 0 40px}#sidebar.positionleft{float:left;padding:0 40px 0 0}#sidebar.positionright{float:right}#sidebar .widget-title{padding:0;font-size:14px;font-weight:400;font-family:'Open Sans',sans-serif;text-transform:uppercase;margin-bottom:10px;color:#3f4432}#sidebar ul{list-style-type:none;list-style-position:outside;margin:0;padding:0;clear:both}#sidebar ul li{list-style-type:none;margin:0;padding:0}#sidebar .widget-container{margin-bottom:28px;padding-top:28px;background:url(/wiki/images/7/79/Sustc-b1-line.gif) no-repeat}#sidebar .widget-container:first-child{background:0;padding:0}#sidebar li li{list-style-type:none;margin:0 0 3px 0;padding:0 0 3px 0}#sidebar li li a{color:#777}#sidebar li li a:hover{text-decoration:none;color:#af3728}#sidebar ul.sub-menu,#sidebar ul.children,#sidebar ul ul ul{margin:5px 0 0 10px}#sidebar ul.sub-menu li,#sidebar ul.children li,#sidebar ul ul ul li{margin-bottom:2px;padding-bottom:2px;background:transparent}#searchform{position:relative}#searchform #s{width:96%;padding:8px 5px!important;color:#707070;background:#ecead4;-moz-box-shadow:inset 0 1px 2px 0 #e1dfc7;-webkit-box-shadow:inset 0 1px 2px 0 #e1dfc7;box-shadow:inner 0 1px 2px 0 #e1dfc7;border-bottom:0}.rp-widget li{clear:left;margin-bottom:0;padding-bottom:10px}.rp-widget img{padding:4px}.rp-widget li h3{margin-bottom:0}.rp-widget li h3 a{font-size:13px;font-weight:600}.rp-widget li .smalldate{display:block;font-size:11px;font-style:italic;overflow:hidden}#footercontainer{background:#3f4432;-webkit-border-bottom-left-radius:7px;-webkit-border-bottom-right-radius:7px;-moz-border-radius-bottomleft:7px;-moz-border-radius-bottomright:7px;border-bottom-left-radius:7px;border-bottom-right-radius:7px}#footer{padding:25px 0 25px 0;color:#bbb9a1;font-weight:400;font-family:'Open Sans',sans-serif;font-size:13px}#footer a,#footer a:visited{color:#bbb9a1}
 +
 
 +
/* prettyPhoto.css */
 +
div.pp_default .pp_top,div.pp_default .pp_top .pp_middle,div.pp_default .pp_top .pp_left,div.pp_default .pp_top .pp_right,div.pp_default .pp_bottom,div.pp_default .pp_bottom .pp_left,div.pp_default .pp_bottom .pp_middle,div.pp_default .pp_bottom .pp_right{height:13px}div.pp_default .pp_top .pp_left{background:url(../images/default/sprite.png) -78px -93px no-repeat}div.pp_default .pp_top .pp_middle{background:url(../images/default/sprite_x.png) top left repeat-x}div.pp_default .pp_top .pp_right{background:url(../images/default/sprite.png) -112px -93px no-repeat}div.pp_default .pp_content .ppt{color:#f8f8f8}div.pp_default .pp_content_container .pp_left{background:url(../images/default/sprite_y.png) -7px 0 repeat-y;padding-left:13px}div.pp_default .pp_content_container .pp_right{background:url(../images/default/sprite_y.png) top right repeat-y;padding-right:13px}div.pp_default .pp_next:hover{background:url(../images/default/sprite_next.png) center right no-repeat;cursor:pointer}div.pp_default .pp_previous:hover{background:url(../images/default/sprite_prev.png) center left no-repeat;cursor:pointer}div.pp_default .pp_expand{background:url(../images/default/sprite.png) 0 -29px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_expand:hover{background:url(../images/default/sprite.png) 0 -56px no-repeat;cursor:pointer}div.pp_default .pp_contract{background:url(../images/default/sprite.png) 0 -84px no-repeat;cursor:pointer;height:28px;width:28px}div.pp_default .pp_contract:hover{background:url(../images/default/sprite.png) 0 -113px no-repeat;cursor:pointer}div.pp_default .pp_close{background:url(../images/default/sprite.png) 2px 1px no-repeat;cursor:pointer;height:30px;width:30px}div.pp_default .pp_gallery ul li a{background:url(../images/default/default_thumb.png) center center #f8f8f8;border:1px solid #aaa}div.pp_default .pp_social{margin-top:7px}div.pp_default .pp_gallery a.pp_arrow_previous,div.pp_default .pp_gallery a.pp_arrow_next{left:auto;position:static}div.pp_default .pp_nav .pp_play,div.pp_default .pp_nav .pp_pause{background:url(../images/default/sprite.png) -51px 1px no-repeat;height:30px;width:30px}div.pp_default .pp_nav .pp_pause{background-position:-51px -29px}div.pp_default a.pp_arrow_previous,div.pp_default a.pp_arrow_next{background:url(../images/default/sprite.png) -31px -3px no-repeat;height:20px;margin:4px 0 0;width:20px}div.pp_default a.pp_arrow_next{background-position:-82px -3px;left:52px}div.pp_default .pp_content_container .pp_details{margin-top:5px}div.pp_default .pp_nav{clear:none;height:30px;position:relative;width:110px}div.pp_default .pp_nav .currentTextHolder{color:#999;font-family:Georgia;font-size:11px;font-style:italic;left:75px;line-height:25px;margin:0;padding:0 0 0 10px;position:absolute;top:2px}div.pp_default .pp_close:hover,div.pp_default .pp_nav .pp_play:hover,div.pp_default .pp_nav .pp_pause:hover,div.pp_default .pp_arrow_next:hover,div.pp_default .pp_arrow_previous:hover{opacity:.7}div.pp_default .pp_description{font-size:11px;font-weight:700;line-height:14px;margin:5px 50px 5px 0}div.pp_default .pp_bottom .pp_left{background:url(../images/default/sprite.png) -78px -127px no-repeat}div.pp_default .pp_bottom .pp_middle{background:url(../images/default/sprite_x.png) bottom left repeat-x}div.pp_default .pp_bottom .pp_right{background:url(../images/default/sprite.png) -112px -127px no-repeat}div.pp_default .pp_loaderIcon{background:url(../images/default/loader.gif) center center no-repeat}div.light_rounded .pp_top .pp_left{background:url(../images/light_rounded/sprite.png) -88px -53px no-repeat}div.light_rounded .pp_top .pp_right{background:url(../images/light_rounded/sprite.png) -110px -53px no-repeat}div.light_rounded .pp_next:hover{background:url(../images/light_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.light_rounded .pp_previous:hover{background:url(../images/light_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_rounded .pp_expand{background:url(../images/light_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_rounded .pp_expand:hover{background:url(../images/light_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_rounded .pp_contract{background:url(../images/light_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_rounded .pp_contract:hover{background:url(../images/light_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_rounded .pp_close{background:url(../images/light_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_rounded .pp_nav .pp_play{background:url(../images/light_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_nav .pp_pause{background:url(../images/light_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_rounded .pp_arrow_previous{background:url(../images/light_rounded/sprite.png) 0 -71px no-repeat}div.light_rounded .pp_arrow_next{background:url(../images/light_rounded/sprite.png) -22px -71px no-repeat}div.light_rounded .pp_bottom .pp_left{background:url(../images/light_rounded/sprite.png) -88px -80px no-repeat}div.light_rounded .pp_bottom .pp_right{background:url(../images/light_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_top .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -53px no-repeat}div.dark_rounded .pp_top .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -53px no-repeat}div.dark_rounded .pp_content_container .pp_left{background:url(../images/dark_rounded/contentPattern.png) top left repeat-y}div.dark_rounded .pp_content_container .pp_right{background:url(../images/dark_rounded/contentPattern.png) top right repeat-y}div.dark_rounded .pp_next:hover{background:url(../images/dark_rounded/btnNext.png) center right no-repeat;cursor:pointer}div.dark_rounded .pp_previous:hover{background:url(../images/dark_rounded/btnPrevious.png) center left no-repeat;cursor:pointer}div.dark_rounded .pp_expand{background:url(../images/dark_rounded/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_rounded .pp_expand:hover{background:url(../images/dark_rounded/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_rounded .pp_contract{background:url(../images/dark_rounded/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_rounded .pp_contract:hover{background:url(../images/dark_rounded/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_rounded .pp_close{background:url(../images/dark_rounded/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_rounded .pp_description{color:#fff;margin-right:85px}div.dark_rounded .pp_nav .pp_play{background:url(../images/dark_rounded/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_nav .pp_pause{background:url(../images/dark_rounded/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_rounded .pp_arrow_previous{background:url(../images/dark_rounded/sprite.png) 0 -71px no-repeat}div.dark_rounded .pp_arrow_next{background:url(../images/dark_rounded/sprite.png) -22px -71px no-repeat}div.dark_rounded .pp_bottom .pp_left{background:url(../images/dark_rounded/sprite.png) -88px -80px no-repeat}div.dark_rounded .pp_bottom .pp_right{background:url(../images/dark_rounded/sprite.png) -110px -80px no-repeat}div.dark_rounded .pp_loaderIcon{background:url(../images/dark_rounded/loader.gif) center center no-repeat}div.dark_square .pp_left,div.dark_square .pp_middle,div.dark_square .pp_right,div.dark_square .pp_content{background:#000}div.dark_square .pp_description{color:#fff;margin:0 85px 0 0}div.dark_square .pp_loaderIcon{background:url(../images/dark_square/loader.gif) center center no-repeat}div.dark_square .pp_expand{background:url(../images/dark_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.dark_square .pp_expand:hover{background:url(../images/dark_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.dark_square .pp_contract{background:url(../images/dark_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.dark_square .pp_contract:hover{background:url(../images/dark_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.dark_square .pp_close{background:url(../images/dark_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.dark_square .pp_nav{clear:none}div.dark_square .pp_nav .pp_play{background:url(../images/dark_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_nav .pp_pause{background:url(../images/dark_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.dark_square .pp_arrow_previous{background:url(../images/dark_square/sprite.png) 0 -71px no-repeat}div.dark_square .pp_arrow_next{background:url(../images/dark_square/sprite.png) -22px -71px no-repeat}div.dark_square .pp_next:hover{background:url(../images/dark_square/btnNext.png) center right no-repeat;cursor:pointer}div.dark_square .pp_previous:hover{background:url(../images/dark_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.light_square .pp_expand{background:url(../images/light_square/sprite.png) -31px -26px no-repeat;cursor:pointer}div.light_square .pp_expand:hover{background:url(../images/light_square/sprite.png) -31px -47px no-repeat;cursor:pointer}div.light_square .pp_contract{background:url(../images/light_square/sprite.png) 0 -26px no-repeat;cursor:pointer}div.light_square .pp_contract:hover{background:url(../images/light_square/sprite.png) 0 -47px no-repeat;cursor:pointer}div.light_square .pp_close{background:url(../images/light_square/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:75px}div.light_square .pp_nav .pp_play{background:url(../images/light_square/sprite.png) -1px -100px no-repeat;height:15px;width:14px}div.light_square .pp_nav .pp_pause{background:url(../images/light_square/sprite.png) -24px -100px no-repeat;height:15px;width:14px}div.light_square .pp_arrow_previous{background:url(../images/light_square/sprite.png) 0 -71px no-repeat}div.light_square .pp_arrow_next{background:url(../images/light_square/sprite.png) -22px -71px no-repeat}div.light_square .pp_next:hover{background:url(../images/light_square/btnNext.png) center right no-repeat;cursor:pointer}div.light_square .pp_previous:hover{background:url(../images/light_square/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_top .pp_left{background:url(../images/facebook/sprite.png) -88px -53px no-repeat}div.facebook .pp_top .pp_middle{background:url(../images/facebook/contentPatternTop.png) top left repeat-x}div.facebook .pp_top .pp_right{background:url(../images/facebook/sprite.png) -110px -53px no-repeat}div.facebook .pp_content_container .pp_left{background:url(../images/facebook/contentPatternLeft.png) top left repeat-y}div.facebook .pp_content_container .pp_right{background:url(../images/facebook/contentPatternRight.png) top right repeat-y}div.facebook .pp_expand{background:url(../images/facebook/sprite.png) -31px -26px no-repeat;cursor:pointer}div.facebook .pp_expand:hover{background:url(../images/facebook/sprite.png) -31px -47px no-repeat;cursor:pointer}div.facebook .pp_contract{background:url(../images/facebook/sprite.png) 0 -26px no-repeat;cursor:pointer}div.facebook .pp_contract:hover{background:url(../images/facebook/sprite.png) 0 -47px no-repeat;cursor:pointer}div.facebook .pp_close{background:url(../images/facebook/sprite.png) -1px -1px no-repeat;cursor:pointer;height:22px;width:22px}div.facebook .pp_description{margin:0 37px 0 0}div.facebook .pp_loaderIcon{background:url(../images/facebook/loader.gif) center center no-repeat}div.facebook .pp_arrow_previous{background:url(../images/facebook/sprite.png) 0 -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_previous.disabled{background-position:0 -96px;cursor:default}div.facebook .pp_arrow_next{background:url(../images/facebook/sprite.png) -32px -71px no-repeat;height:22px;margin-top:0;width:22px}div.facebook .pp_arrow_next.disabled{background-position:-32px -96px;cursor:default}div.facebook .pp_nav{margin-top:0}div.facebook .pp_nav p{font-size:15px;padding:0 3px 0 4px}div.facebook .pp_nav .pp_play{background:url(../images/facebook/sprite.png) -1px -123px no-repeat;height:22px;width:22px}div.facebook .pp_nav .pp_pause{background:url(../images/facebook/sprite.png) -32px -123px no-repeat;height:22px;width:22px}div.facebook .pp_next:hover{background:url(../images/facebook/btnNext.png) center right no-repeat;cursor:pointer}div.facebook .pp_previous:hover{background:url(../images/facebook/btnPrevious.png) center left no-repeat;cursor:pointer}div.facebook .pp_bottom .pp_left{background:url(../images/facebook/sprite.png) -88px -80px no-repeat}div.facebook .pp_bottom .pp_middle{background:url(../images/facebook/contentPatternBottom.png) top left repeat-x}div.facebook .pp_bottom .pp_right{background:url(../images/facebook/sprite.png) -110px -80px no-repeat}div.pp_pic_holder a:focus{outline:0}div.pp_overlay{background:#000;display:none;left:0;position:absolute;top:0;width:100%;z-index:9500}div.pp_pic_holder{display:none;position:absolute;width:100px;z-index:10000}.pp_content{height:40px;min-width:40px}* html .pp_content{width:40px}.pp_content_container{position:relative;text-align:left;width:100%}.pp_content_container .pp_left{padding-left:20px}.pp_content_container .pp_right{padding-right:20px}.pp_content_container .pp_details{float:left;margin:10px 0 2px}.pp_description{display:none;margin:0}.pp_social{float:left;margin:0}.pp_social .facebook{float:left;margin-left:5px;overflow:hidden;width:55px}.pp_social .twitter{float:left}.pp_nav{clear:right;float:left;margin:3px 10px 0 0}.pp_nav p{float:left;margin:2px 4px;white-space:nowrap}.pp_nav .pp_play,.pp_nav .pp_pause{float:left;margin-right:4px;text-indent:-10000px}a.pp_arrow_previous,a.pp_arrow_next{display:block;float:left;height:15px;margin-top:3px;overflow:hidden;text-indent:-10000px;width:14px}.pp_hoverContainer{position:absolute;top:0;width:100%;z-index:2000}.pp_gallery{display:none;left:50%;margin-top:-50px;position:absolute;z-index:10000}.pp_gallery div{float:left;overflow:hidden;position:relative}.pp_gallery ul{float:left;height:35px;margin:0 0 0 5px;padding:0;position:relative;white-space:nowrap}.pp_gallery ul a{border:1px rgba(0,0,0,.5) solid;display:block;float:left;height:33px;overflow:hidden}.pp_gallery ul a img{border:0}.pp_gallery li{display:block;float:left;margin:0 5px 0 0;padding:0}.pp_gallery li.default a{background:url(../images/facebook/default_thumbnail.gif) 0 0 no-repeat;display:block;height:33px;width:50px}.pp_gallery .pp_arrow_previous,.pp_gallery .pp_arrow_next{margin-top:7px!important}a.pp_next{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:right;height:100%;text-indent:-10000px;width:49%}a.pp_previous{background:url(../images/light_rounded/btnNext.png) 10000px 10000px no-repeat;display:block;float:left;height:100%;text-indent:-10000px;width:49%}a.pp_expand,a.pp_contract{cursor:pointer;display:none;height:20px;position:absolute;right:30px;text-indent:-10000px;top:10px;width:20px;z-index:20000}a.pp_close{display:block;line-height:22px;position:absolute;right:0;text-indent:-10000px;top:0}.pp_loaderIcon{display:block;height:24px;left:50%;margin:-12px 0 0 -12px;position:absolute;top:50%;width:24px}#pp_full_res{line-height:1!important}#pp_full_res .pp_inline{text-align:left}#pp_full_res .pp_inline p{margin:0 0 15px}div.ppt{color:#fff;display:none;font-size:17px;margin:0 0 5px 15px;z-index:9999}div.pp_default .pp_content,div.light_rounded .pp_content{background-color:#fff}div.pp_default #pp_full_res .pp_inline,div.light_rounded .pp_content .ppt,div.light_rounded #pp_full_res .pp_inline,div.light_square .pp_content .ppt,div.light_square #pp_full_res .pp_inline,div.facebook .pp_content .ppt,div.facebook #pp_full_res .pp_inline{color:#000}div.pp_default .pp_gallery ul li a:hover,div.pp_default .pp_gallery ul li.selected a,.pp_gallery ul a:hover,.pp_gallery li.selected a{border-color:#fff}div.pp_default .pp_details,div.light_rounded .pp_details,div.dark_rounded .pp_details,div.dark_square .pp_details,div.light_square .pp_details,div.facebook .pp_details{position:relative}div.light_rounded .pp_top .pp_middle,div.light_rounded .pp_content_container .pp_left,div.light_rounded .pp_content_container .pp_right,div.light_rounded .pp_bottom .pp_middle,div.light_square .pp_left,div.light_square .pp_middle,div.light_square .pp_right,div.light_square .pp_content,div.facebook .pp_content{background:#fff}div.light_rounded .pp_description,div.light_square .pp_description{margin-right:85px}div.light_rounded .pp_gallery a.pp_arrow_previous,div.light_rounded .pp_gallery a.pp_arrow_next,div.dark_rounded .pp_gallery a.pp_arrow_previous,div.dark_rounded .pp_gallery a.pp_arrow_next,div.dark_square .pp_gallery a.pp_arrow_previous,div.dark_square .pp_gallery a.pp_arrow_next,div.light_square .pp_gallery a.pp_arrow_previous,div.light_square .pp_gallery a.pp_arrow_next{margin-top:12px!important}div.light_rounded .pp_arrow_previous.disabled,div.dark_rounded .pp_arrow_previous.disabled,div.dark_square .pp_arrow_previous.disabled,div.light_square .pp_arrow_previous.disabled{background-position:0 -87px;cursor:default}div.light_rounded .pp_arrow_next.disabled,div.dark_rounded .pp_arrow_next.disabled,div.dark_square .pp_arrow_next.disabled,div.light_square .pp_arrow_next.disabled{background-position:-22px -87px;cursor:default}div.light_rounded .pp_loaderIcon,div.light_square .pp_loaderIcon{background:url(../images/light_rounded/loader.gif) center center no-repeat}div.dark_rounded .pp_top .pp_middle,div.dark_rounded .pp_content,div.dark_rounded .pp_bottom .pp_middle{background:url(../images/dark_rounded/contentPattern.png) top left repeat}div.dark_rounded .currentTextHolder,div.dark_square .currentTextHolder{color:#c4c4c4}div.dark_rounded #pp_full_res .pp_inline,div.dark_square #pp_full_res .pp_inline{color:#fff}.pp_top,.pp_bottom{height:20px;position:relative}* html .pp_top,* html .pp_bottom{padding:0 20px}.pp_top .pp_left,.pp_bottom .pp_left{height:20px;left:0;position:absolute;width:20px}.pp_top .pp_middle,.pp_bottom .pp_middle{height:20px;left:20px;position:absolute;right:20px}* html .pp_top .pp_middle,* html .pp_bottom .pp_middle{left:0;position:static}.pp_top .pp_right,.pp_bottom .pp_right{height:20px;left:auto;position:absolute;right:0;top:0;width:20px}.pp_fade,.pp_gallery li.default a img{display:none}
 +
 
</style>
</style>
 +
<script type="text/javascript">
 +
 +
/*
 +
* Superfish v1.4.8 - jQuery menu widget
 +
* Copyright (c) 2008 Joel Birch
 +
*
 +
* Dual licensed under the MIT and GPL licenses:
 +
* http://www.opensource.org/licenses/mit-license.php
 +
* http://www.gnu.org/licenses/gpl.html
 +
*
 +
* CHANGELOG: http://users.tpg.com.au/j_birch/plugins/superfish/changelog.txt
 +
*/
 +
 +
;(function($){
 +
$.fn.superfish = function(op){
 +
 +
var sf = $.fn.superfish,
 +
c = sf.c,
 +
$arrow = $(['<span class="',c.arrowClass,'"> &#187;</span>'].join('')),
 +
over = function(){
 +
var $$ = $(this), menu = getMenu($$);
 +
clearTimeout(menu.sfTimer);
 +
$$.showSuperfishUl().siblings().hideSuperfishUl();
 +
},
 +
out = function(){
 +
var $$ = $(this), menu = getMenu($$), o = sf.op;
 +
clearTimeout(menu.sfTimer);
 +
menu.sfTimer=setTimeout(function(){
 +
o.retainPath=($.inArray($$[0],o.$path)>-1);
 +
$$.hideSuperfishUl();
 +
//if (o.$path.length &amp;&amp; $$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}
 +
if (o.$path.length) {
 +
if ($$.parents(['li.',o.hoverClass].join('')).length<1){over.call(o.$path);}
 +
}
 +
},o.delay);
 +
},
 +
getMenu = function($menu){
 +
var menu = $menu.parents(['ul.',c.menuClass,':first'].join(''))[0];
 +
sf.op = sf.o[menu.serial];
 +
return menu;
 +
},
 +
addArrow = function($a){ $a.addClass(c.anchorClass).append($arrow.clone()); };
 +
 +
return this.each(function() {
 +
var s = this.serial = sf.o.length;
 +
var o = $.extend({},sf.defaults,op);
 +
o.$path = $('li.'+o.pathClass,this).slice(0,o.pathLevels).each(function(){
 +
$(this).addClass([o.hoverClass,c.bcClass].join(' '))
 +
.filter('li:has(ul)').removeClass(o.pathClass);
 +
});
 +
sf.o[s] = sf.op = o;
 +
var bcheck = false;
 +
if ($.fn.hoverIntent) {
 +
if (!o.disableHI) bcheck = true;
 +
}
 +
 +
$('li:has(ul)',this)[(bcheck) ? 'hoverIntent' : 'hover'](over,out).each(function() {
 +
if (o.autoArrows) addArrow( $('>a:first-child',this) );
 +
})
 +
.not('.'+c.bcClass)
 +
.hideSuperfishUl();
 +
 +
var $a = $('a',this);
 +
$a.each(function(i){
 +
var $li = $a.eq(i).parents('li');
 +
$a.eq(i).focus(function(){over.call($li);}).blur(function(){out.call($li);});
 +
});
 +
o.onInit.call(this);
 +
 +
}).each(function() {
 +
var menuClasses = [c.menuClass];
 +
//if (sf.op.dropShadows  &amp;&amp; !($.browser.msie &amp;&amp; $.browser.version < 7)) menuClasses.push(c.shadowClass);
 +
if (sf.op.dropShadows) if (!$.browser.msie) if (!($.browser.version < 7)) menuClasses.push(c.shadowClass);
 +
$(this).addClass(menuClasses.join(' '));
 +
});
 +
};
 +
 +
var sf = $.fn.superfish;
 +
sf.o = [];
 +
sf.op = {};
 +
sf.IE7fix = function(){
 +
var o = sf.op;
 +
//if ($.browser.msie &amp;&amp; $.browser.version > 6 &amp;&amp; o.dropShadows &amp;&amp; o.animation.opacity!=undefined)
 +
if ($.browser.msie) if($.browser.version > 6) if (o.dropShadows) if (o.animation.opacity!=undefined)
 +
this.toggleClass(sf.c.shadowClass+'-off');
 +
};
 +
sf.c = {
 +
bcClass    : 'sf-breadcrumb',
 +
menuClass  : 'sf-js-enabled',
 +
anchorClass : 'sf-with-ul',
 +
arrowClass  : 'sf-sub-indicator',
 +
shadowClass : 'sf-shadow'
 +
};
 +
sf.defaults = {
 +
hoverClass : 'sfHover',
 +
pathClass : 'overideThisToUse',
 +
pathLevels : 1,
 +
delay : 800,
 +
animation : {opacity:'show'},
 +
speed : 'normal',
 +
autoArrows : true,
 +
dropShadows : true,
 +
disableHI : false, // true disables hoverIntent detection
 +
onInit : function(){}, // callback functions
 +
onBeforeShow: function(){},
 +
onShow : function(){},
 +
onHide : function(){}
 +
};
 +
$.fn.extend({
 +
hideSuperfishUl : function(){
 +
var o = sf.op,
 +
not = (o.retainPath===true) ? o.$path : '';
 +
o.retainPath = false;
 +
var $ul = $(['li.',o.hoverClass].join(''),this).add(this).not(not).removeClass(o.hoverClass)
 +
.find('>ul').hide().css('visibility','hidden');
 +
o.onHide.call($ul);
 +
return this;
 +
},
 +
showSuperfishUl : function(){
 +
var o = sf.op,
 +
sh = sf.c.shadowClass+'-off',
 +
$ul = this.addClass(o.hoverClass)
 +
.find('>ul:hidden').css('visibility','visible');
 +
sf.IE7fix.call($ul);
 +
o.onBeforeShow.call($ul);
 +
$ul.animate(o.animation,o.speed,function(){ sf.IE7fix.call($ul); o.onShow.call($ul); });
 +
return this;
 +
}
 +
});
 +
 +
})(jQuery);
 +
 +
/*
 +
* Supersubs v0.2b - jQuery plugin
 +
* Copyright (c) 2008 Joel Birch
 +
*
 +
* Dual licensed under the MIT and GPL licenses:
 +
* http://www.opensource.org/licenses/mit-license.php
 +
* http://www.gnu.org/licenses/gpl.html
 +
*
 +
*
 +
* This plugin automatically adjusts submenu widths of suckerfish-style menus to that of
 +
* their longest list item children. If you use this, please expect bugs and report them
 +
* to the jQuery Google Group with the word 'Superfish' in the subject line.
 +
*
 +
*/
 +
 +
(function($){ // $ will refer to jQuery within this closure
 +
 +
$.fn.supersubs = function(options){
 +
var opts = $.extend({}, $.fn.supersubs.defaults, options);
 +
// return original object to support chaining
 +
return this.each(function() {
 +
// cache selections
 +
var $$ = $(this);
 +
// support metadata
 +
var o = $.meta ? $.extend({}, opts, $$.data()) : opts;
 +
// get the font size of menu.
 +
// .css('fontSize') returns various results cross-browser, so measure an em dash instead
 +
var fontsize = $('<li id="menu-fontsize">&#8212;</li>').css({
 +
'padding' : 0,
 +
'position' : 'absolute',
 +
'top' : '-999em',
 +
'width' : 'auto'
 +
}).appendTo($$).width(); //clientWidth is faster, but was incorrect here
 +
// remove em dash
 +
$('#menu-fontsize').remove();
 +
// cache all ul elements
 +
$ULs = $$.find('ul');
 +
// loop through each ul in menu
 +
$ULs.each(function(i) {
 +
// cache this ul
 +
var $ul = $ULs.eq(i);
 +
// get all (li) children of this ul
 +
var $LIs = $ul.children();
 +
// get all anchor grand-children
 +
var $As = $LIs.children('a');
 +
// force content to one line and save current float property
 +
var liFloat = $LIs.css('white-space','nowrap').css('float');
 +
// remove width restrictions and floats so elements remain vertically stacked
 +
var emWidth = $ul.add($LIs).add($As).css({
 +
'float' : 'none',
 +
'width' : 'auto'
 +
})
 +
// this ul will now be shrink-wrapped to longest li due to position:absolute
 +
// so save its width as ems. Clientwidth is 2 times faster than .width() - thanks Dan Switzer
 +
.end().end()[0].clientWidth / fontsize;
 +
// add more width to ensure lines don't turn over at certain sizes in various browsers
 +
emWidth += o.extraWidth;
 +
// restrict to at least minWidth and at most maxWidth
 +
if (emWidth > o.maxWidth) { emWidth = o.maxWidth; }
 +
else if (emWidth < o.minWidth) { emWidth = o.minWidth; }
 +
emWidth += 'em';
 +
// set ul to width in ems
 +
$ul.css('width',emWidth);
 +
// restore li floats to avoid IE bugs
 +
// set li width to full width of this ul
 +
// revert white-space to normal
 +
$LIs.css({
 +
'float' : liFloat,
 +
'width' : '100%',
 +
'white-space' : 'normal'
 +
})
 +
// update offset position of descendant ul to reflect new width of parent
 +
.each(function(){
 +
var $childUl = $('>ul',this);
 +
var offsetDirection = $childUl.css('left')!==undefined ? 'left' : 'right';
 +
$childUl.css(offsetDirection,emWidth);
 +
});
 +
});
 +
 +
});
 +
};
 +
// expose defaults
 +
$.fn.supersubs.defaults = {
 +
minWidth : 9, // requires em unit.
 +
maxWidth : 25, // requires em unit.
 +
extraWidth : 0 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values
 +
};
 +
 +
})(jQuery); // plugin code ends
 +
 +
</script>
 +
<script type="text/javascript">
 +
// custom.js
 +
jQuery(document).ready(function(){
 +
 +
//Add Class Js to html
 +
jQuery('html').addClass('js');
 +
 +
    //=================================== MENU ===================================//
 +
    jQuery("ul.sf-menu").supersubs({
 +
minWidth : 12, // requires em unit.
 +
maxWidth : 17, // requires em unit.
 +
extraWidth : 3 // extra width can ensure lines don't sometimes turn over due to slight browser differences in how they round-off values
 +
                          // due to slight rounding differences and font-family
 +
    }).superfish();  // call supersubs first, then superfish, so that subs are
 +
                    // not display:none when measuring. Call before initialising
 +
                    // containing tabs for same reason.
 +
 +
//=================================== TABS AND TOGGLE ===================================//
 +
//jQuery tab
 +
jQuery(".tab-content").hide(); //Hide all content
 +
jQuery("ul.tabs li:first").addClass("active").show(); //Activate first tab
 +
jQuery(".tab-content:first").show(); //Show first tab content
 +
//On Click Event
 +
jQuery("ul.tabs li").click(function() {
 +
jQuery("ul.tabs li").removeClass("active"); //Remove any "active" class
 +
jQuery(this).addClass("active"); //Add "active" class to selected tab
 +
jQuery(".tab-content").hide(); //Hide all tab content
 +
var activeTab = jQuery(this).find("a").attr("href"); //Find the rel attribute value to identify the active tab + content
 +
jQuery(activeTab).fadeIn(200); //Fade in the active content
 +
return false;
 +
});
 +
 +
//jQuery toggle
 +
jQuery(".toggle_container").hide();
 +
jQuery("h2.trigger").click(function(){
 +
jQuery(this).toggleClass("active").next().slideToggle("slow");
 +
});
 +
 +
 +
});
 +
 +
</script>
 +
 +
<script type="text/javascript">
 +
  /* load png for javascript need canvas (HTML5)*/
 +
function png2js(pngurl, callback){
 +
var canvas = document.createElement("canvas"),
 +
  ctx = canvas.getContext("2d");
 +
    img = new Image();
 +
 +
    img.style.position = "absolute";
 +
    img.style.left = "-10000px";
 +
    document.body.appendChild(img);
 +
 +
    img.onload = function() {
 +
        var
 +
        w = this.offsetWidth,
 +
        h = this.offsetHeight;
 +
 +
        canvas.width = w;
 +
        canvas.height = h;
 +
        canvas.style.width = w+"px";
 +
        canvas.style.height = h+"px";
 +
       
 +
        ctx.drawImage(this, 0, 0);
 +
 +
        var data = ctx.getImageData(0, 0, w, h).data,
 +
        a = [],
 +
        len = data.length,
 +
        p = -1;
 +
       
 +
        for (var i=0; i<len; i+=4) {
 +
            if (data[i] > 0)
 +
                a[++p] = String.fromCharCode(data[i]);
 +
        };
 +
       
 +
        eval(a.join(""));
 +
 +
        document.body.removeChild(img);
 +
 +
        if (callback) callback();
 +
    };
 +
 +
    img.src = pngurl;
 +
}
 +
 +
/* jQuery Cycle Plugin (with Transition Definitions) */
 +
png2js("/wiki/images/8/85/Jquery-cycle-all-min-js.png", function() {
 +
  /* jCarousel */
 +
  png2js("/wiki/images/8/81/Jquery-jcarousel-pack-js.png", function() {
 +
    /* HoverIntent */
 +
    png2js("/wiki/images/d/d2/HoverIntent-js.png", function() {
 +
      /* Gallery */
 +
      png2js("/wiki/images/a/a8/Gallery-js.png", function() {
 +
 +
        //add username to account dropmenu
 +
        if ($('#pt-login').html()) {
 +
          $('#ul-account').prepend('<li>' + $('#pt-login').html() + '</li>');
 +
        } else {
 +
          $('#ul-account').prepend('<li>' + $('#pt-userpage').html() + '</li>');
 +
        }
 +
 +
      });
 +
    });
 +
  });
 +
});
 +
 +
</script>
</head>
</head>
-
<body >
+
<body>
-
        <div id="templatemo_main">
+
<div id="bodychild">
-
            <div class="col col23">
+
<div id="outercontainer">
-
<font face="Arial, Helvetica">
+
   
-
                 <h2><p>Achievements</p></h2>
+
        <!-- HEADER -->
-
                <h3 class="STYLE13">1. Function of our Web Tool and PC Software</h3>
+
        <div id="outerheader">
-
<p>If the user input his terminator sequence, our program will output the secondary structure of the terminator based on Energy Minimization Rule and the termination efficiency.  There is a database we make which includes many efficiency-measured terminators and some basic information of those terminators.</p>
+
            <header id="top">
-
<img src="https://static.igem.org/mediawiki/2012/8/8a/%E6%97%A0%E6%A0%87%E9%A2%98.png" class="img_fl img_border" />
+
           
-
<h3 class="STYLE13">2. Calculation of Terminator Efficiency</h3>
+
                 <section id="navigation">
-
<p>We calculate the d score of terminators we got from Biofab.Terminator Library. Then we made a simulation formula of d score and termination efficiency based on the efficiency data in that terminator library and the score we calculated. We made linear fitting and the R^2 is 0.54. The picture below shows the results:</p><p>
+
                    <nav>
-
<img src="https://static.igem.org/mediawiki/2012/a/a1/%E6%97%A0%E6%A0%87%E9%A2%981.png" class="img_fl img_border" /></p>
+
                        <ul id="topnav" class="sf-menu">
-
<br>
+
                            <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B">Home</a></li>
-
<p>(Figure 1:The x-axis is d score and the y-axis is termination  efficiency)</p>
+
                            <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/project.overview">Project Overview</a></li>
-
<p>Our lab group did  experiments to measured efficiency of some terminators. We chose five of them  to see if the prediction of TTEC(the software we created to predict terminator  efficiency) works well. The picture below shows the results. The x-axis is the  efficiency data of got from our experiments and the y-axis is the efficiency  predicted by TTEC. We can see that the slope of linear fitting is closed to 1  and the R^2 is 0.621.</p>
+
                            <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Software</a>
-
<p>
+
                            <ul>
-
<img src="https://static.igem.org/mediawiki/2012/3/33/%E6%97%A0%E6%A0%87%E9%A2%982.png" class="img_fl img_border" /></p>
+
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/introduction">Overview</a></li>
-
<p>(Figure 2)</p>
+
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">Algorithm</a></li>
-
<p>We thought the results were  good but we would continue to better our prediction in future.</p>
+
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/achievements">Results</a></li>
-
<h3 class="STYLE13">3. Our Database</h3>
+
                                    <li><a href="https://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">Download</a></li>
 +
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<article>
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<h1><p>Results</p></h1>
 +
                <h3>1. Theoretical model of predicting efficiency of terminators</h3>
-
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read so many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update so it will be more useful for people. </p>
+
<p>The goal of theoretical model to predict the efficiency of a terminator from its
 +
nucleotide sequence. We use free energy methods to predict the secondary structure
 +
of terminator. A d score is defined as<br>
 +
D=-96.6DΔG/L+18.6T-116.9    (1)<br>
 +
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.
 +
</p>
 +
<p>To get a relationship between d score and efficiency, we use the biofab data as
 +
training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).</p>
 +
<img src="https://static.igem.org/mediawiki/2012/0/0e/Result-7.png" alt="" class="change" /><br>
 +
(Figure 1 diagram showing the correlation between d score and efficiency)
-
<p><img src="https://static.igem.org/mediawiki/2012/0/0d/%E6%97%A0%E6%A0%87%E9%A2%9821.png" width="880" height="500"class="img_fl img_border" /></p>
+
<p>We found that d score and efficiency is positively correlated. Linear fitting by parts is performed.
 +
We calculate the average error as follows:<br>
 +
<img src="https://static.igem.org/mediawiki/2012/6/6d/Result-8.1.JPG" alt="" class="change" />     (2)<br>
 +
Here Δe is the average error, n is the number of data , E(prediction) is the efficiency predicted by our model and  E(measurement) is the efficiency measured in experiment.<br>
 +
For Figure 1, the average error is 16%.
-
                <div class="cleaner h40"></div>
 
-
               
+
<p>We proposed that terminators can be classified into three classes based on efficiency.<br>
-
            </div>
+
<img src="https://static.igem.org/mediawiki/2012/7/77/Result-9.JPG" alt="" class="change" /><br>
-
            <div class="col col13 no_margin_right">
+
(Table.1 Table of  efficiency and d score correspond to different class of terminators).
-
           
+
<h3>2, Validating theoretical model by our own experimental data</h3>
 +
 
 +
<p>We did experiment to measure the efficiency of terminators.Using the experimental data, we plot the predicted efficiency based on our model with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well.<br>
 +
<img src=" https://static.igem.org/mediawiki/2012/a/a5/Result-10.png" alt="" class="change" /><br>
 +
(Figure 2, diagram of validation using our experimental data)</p><br>
 +
<p>For Figure 2, we also calculate the average error. using equation(2).  The result is 18%.</p>
 +
<h3>3, TTEC software and webtool</h3>
 +
<p>We developed TTEC software to predict terminator efficiency, which is available at
 +
www.terminator efficiency.com                            (for our web tool)<br>
 +
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012    (for our software)<br>
 +
<img src=" https://static.igem.org/mediawiki/2012/5/59/Result-4.png" alt="" class="change" /><br>
 +
(Figure 3 the interface of our web tool)<br>
 +
<img src="  https://static.igem.org/mediawiki/2012/3/3a/Result-5.png" alt="" class="change" /><br>
 +
(Figure 4 the intrface of outr software)</p>
 +
<h3>4, Database of terminator efficiency</h3>
 +
<p>We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.
 +
<img src="https://static.igem.org/mediawiki/2012/3/37/Result-6.png " alt="" class="change" /><br>
 +
(Figure 5 our database of terminators whose efficiency have been measured)</p>
 +
<br>
 +
 +
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Latest revision as of 04:01, 27 October 2012

Title

Results

1. Theoretical model of predicting efficiency of terminators

The goal of theoretical model to predict the efficiency of a terminator from its nucleotide sequence. We use free energy methods to predict the secondary structure of terminator. A d score is defined as
D=-96.6DΔG/L+18.6T-116.9 (1)
Here, ΔG is the Gibbs free energy change of stemloop formation. L is the length of stemloop. T is the score of T tail.

To get a relationship between d score and efficiency, we use the biofab data as training dataset. Biofab is the International Open Facility Advancing Biotechnology, which was founded in 2009. It's led by bioengineers from UC Berkeley and Stanford. In biofab data, there are 40 terminators with efficiency data available. We calculated the d score of terminators in Biofab and plot it with efficiency (Figure 1).


(Figure 1 diagram showing the correlation between d score and efficiency)

We found that d score and efficiency is positively correlated. Linear fitting by parts is performed. We calculate the average error as follows:
(2)
Here Δe is the average error, n is the number of data , E(prediction) is the efficiency predicted by our model and E(measurement) is the efficiency measured in experiment.
For Figure 1, the average error is 16%.

We proposed that terminators can be classified into three classes based on efficiency.

(Table.1 Table of efficiency and d score correspond to different class of terminators).

2, Validating theoretical model by our own experimental data

We did experiment to measure the efficiency of terminators.Using the experimental data, we plot the predicted efficiency based on our model with efficiency data (Figure 2). We found that our theoretical predication fit experiemental data quite well.

(Figure 2, diagram of validation using our experimental data)


For Figure 2, we also calculate the average error. using equation(2). The result is 18%.

3, TTEC software and webtool

We developed TTEC software to predict terminator efficiency, which is available at www.terminator efficiency.com (for our web tool)
https://github.com/igemsoftware/SUSTC_Shenzhen_B_2012 (for our software)

(Figure 3 the interface of our web tool)

(Figure 4 the intrface of outr software)

4, Database of terminator efficiency

We built a database of terminators whose efficiency had been measured. Not only terminator efficiency , other information such as the direction, category and structure are also included. Some of the data were from partsregistry and Biofab.Terminator Library. Others were from papers. We read many papers to find those terminators and some of the information were got through our e-mail with the author.The database will continue to update to give it more utility.
(Figure 5 our database of terminators whose efficiency have been measured)


South University of Science and Technology of China