Team:SUSTC-Shenzhen-B

From 2012.igem.org

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                                 <h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1>
                                 <h1>Transcriptional Terminator Efficiency Calculator (TTEC)</h1>
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<p>We studied the rho-independent transcription terminators which regulate gene expression using both  
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<p>We used both  
<a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm"> theoretical model</a> and
<a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm"> theoretical model</a> and
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<a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction">experiment</a> method.</p>
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<a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/lab_introduction">experiment</a> method.
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<p> We first developed a theoretical model. This model calculated the free energy of RNA folding and could predict the secondary structure of terminators. From the secondary structure, we proposed an <a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">algorithm </a> that can predict terminator efficiency.</p>
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to study the rho-independent transcription terminators which regulate gene expression.</p>
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<p> To validate our theoretical prediction, we also perform wetlab experiments. We construct 100 terminators. We measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.</p>
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<p> First, we developed a theoretical model to calculate the free energy of RNA folding and predict the secondary structure of terminators. An <a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/algorithm">algorithm </a> was proposed to predict terminator efficiency from the secondary structure.</p>
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<p> We also developed a <a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">software</a> and a <a href="http://www.terminatorefficiency.com/"> web server</a> for people to calculate their terminators and built a
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<p>We also constructed 100 terminators and did some wetlab experiments to validate our theoretical prediction. The basic method was measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.</p>
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<p>Besides,we developed a <a href="http://2012.igem.org/Team:SUSTC-Shenzhen-B/Download">software</a> and a <a href="http://www.terminatorefficiency.com/"> web server</a> for people to calculate terminator efficiency. We also built a  
<a href="http://www.terminatorefficiency.com/Database.html">database</a>
<a href="http://www.terminatorefficiency.com/Database.html">database</a>
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of terminator efficiency which we believe to be the largest database of such kind.</p>
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  of terminator efficiency which we believe is the largest database of such kind. </p>
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<p>Hope our work is useful for the comprehensive study on terminator efficiency.</p>
                 </article>
                 </article>

Revision as of 16:08, 26 October 2012

Title

Transcriptional Terminator Efficiency Calculator (TTEC)

We used both theoretical model and experiment method. to study the rho-independent transcription terminators which regulate gene expression.

First, we developed a theoretical model to calculate the free energy of RNA folding and predict the secondary structure of terminators. An algorithm was proposed to predict terminator efficiency from the secondary structure.

We also constructed 100 terminators and did some wetlab experiments to validate our theoretical prediction. The basic method was measure the the terminator efficiency by measuring the GFP and RFP which are placed before and after terminators.

Besides,we developed a software and a web server for people to calculate terminator efficiency. We also built a database of terminator efficiency which we believe is the largest database of such kind.

Hope our work is useful for the comprehensive study on terminator efficiency.

South University of Science and Technology of China

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