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Team:Penn State/Notebook
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Penn State Notebook
Monday, May 22, 2012
We prepared ampicillin and streptomycin plates in preparation for production of plasmids and competent cells.
Tuesday, May 23, 2012
Inoculation of LB broth with DH10B E. coli strain to prepare master mix/culture of cells and in preparation for making chemically competent cells. These cells were incubated throughout the day and prepared for competency by the end of the day. Promoters were also planned out for the Multiple Promoters Project and ordered. These promoters were designed to combine some of the fluorescent components of the plasmid.
Wednesday, May 24, 2012
Biobricks were hydrated and transformed into Chemically Competent cells through heat shock (
BBa_R0063,
BBa_R1062,
BBa_R0078,
BBa_I1051,
BBa_R0079,
BBa_R0071
). LOB broth was also prepared for the recovery from the transformation.
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