Team:Penn State/Bidirectional Promoters Results

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   <div id="desc">
   <div id="desc">
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     <h3>Team Projects</h3>
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     <h3>Bidirectional Promoters</h3></br>
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     <p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce
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     <p>Utilizing synthetic biology cloning techniques, the Penn State iGEM team has designed three genetic constructs that test the most basic assumptions of molecular biology.
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the expected proteins. Some bidirectional promoters are known to exist, but which way they promote
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While each of the individual projects looks at a different aspect of the mantra, they all come together under the umbrella of Questioning the Central Dogma of Molecular Biology.</p>
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and the degree of expression has not been quantified. This project will test the directionality of  
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    <p class="right"><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></div>
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several BioBrick promoters to answer these questions. </p>
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  </div>
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   <div id="main">
   <div id="main">
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    <h2>Bidirectional Promoters</h2>
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<h3>Palindromic Promoter Circuits</h3>
 +
<p><img src="http://2012.igem.org/wiki/images/f/fc/BiDirresults.png"></p>
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<p>The graph above represents the relative fluorescence measured in a TECANM1000 instrument of several constructs containing various promoter inserts. The reporters indicate the directionality of each promoter sequence measured in the construct. The single biobrick ligated into the construct shows almost complete preference for the forward direction, while three palindromic sequences designed and ligated in the construct demonstrate varying amounts of bidirectionality within the DH10B E.coli cell. Bidirectional promoters are possible within the cell, and those designing promoters should be beware of palindromic sequences in their design and their potential effects on the initiation of protein within the cell. </p>
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    <div id="boxLeft"><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons"><h3>Multiple Start Codons</h3></a>
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<h3>Further Application</h3>
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      <div id="left"><img src="http://2012.igem.org/wiki/images/2/23/Msc.jpg"></div>     
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<p>Further characterization of both biobrick promoters and palindromic sequences will yield more information of the prevalence of bidirectionality in promoters. To view the sequences we plan to test in this construct, please visit our <a href="http://2012.igem.org/Team:Penn_State/Parts">parts</a> page.</p>
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      <p class="block">mRNA strands should have only one recognized start codon. What happens when multiple start codons are introduced in translation?</p>
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<p class="readmore"><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">read more...</a></p>                               
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    <div id="boxCenter"><a href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters"><h3>Bidirectional Promoters</h3></a> 
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      <div id="center"><img src="http://2012.igem.org/wiki/images/e/e1/Bidir.jpg"></div>     
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      <p class="block">Promoters usually initiate translation in one direction. Which promoters promote both foward and reverse on the mRNA strand?
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<p class="readmore"><a href="http://2012.igem.org/Team:Penn_State/Bidirectional_Promoters">read more...</a></p>
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    <div id="boxRight"><a href="http://2012.igem.org/Team:Penn_State/Codon_Optimization"><h3>Codon Optimization</h3></a>
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    <div id="right"><img src="http://2012.igem.org/wiki/images/7/7a/Codopt.jpg"></div></p
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      <p class="block">Each organism has a preferred sequence for each amino acid. What happens when the cell is overloaded by one amino acid codon?<p class="readmore">
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<a href="http://2012.igem.org/Team:Penn_State/Codon_Optimization">read more...</a></p>
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   <div id="sidebar">
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     <h3>Check these Out!</h3>
     <h3>Check these Out!</h3>
     <ul class="sidelink">
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       <li><a href="http://2012.igem.org/Team:Penn_State/Judgin_Criteria">Judging Criteria</a></li>
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       <li><a href="http://2012.igem.org/Team:Penn_State/Judging_Criteria">Judging Criteria</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Human_Practices">Human Practices</a></li>
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    <h3>Picture Gallery</h3>
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    <h3>Projects</h3>
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<h3>Projects</h3>
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     <ul>
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       <li><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li>
       <li><a href="http://2012.igem.org/Team:Penn_State/Multiple_Start_Codons">Multiple Start Codons</a></li>
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     <p><strong>&copy; Hannah Jepsen-Burger<a href="#"></a></strong> | Follow us on Twitter<a href="#"></a></p>
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     <p><strong>&copy; Hannah Jepsen-Burger</strong> | Follow us on Twitter<a href="#"></a></p>
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Latest revision as of 03:53, 27 October 2012

Bidirectional Promoters Overview

Bidirectional Promoters


Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce the expected proteins. Some bidirectional promoters are known to exist, but which way they promote and the degree of expression has not been quantified. This project will test the directionality of several BioBrick promoters to answer these questions.

Bidirectional Promoters

Sample navigation menu:

Overview | Design | Results

Palindromic Promoter Circuits

The graph above represents the relative fluorescence measured in a TECANM1000 instrument of several constructs containing various promoter inserts. The reporters indicate the directionality of each promoter sequence measured in the construct. The single biobrick ligated into the construct shows almost complete preference for the forward direction, while three palindromic sequences designed and ligated in the construct demonstrate varying amounts of bidirectionality within the DH10B E.coli cell. Bidirectional promoters are possible within the cell, and those designing promoters should be beware of palindromic sequences in their design and their potential effects on the initiation of protein within the cell.

Further Application

Further characterization of both biobrick promoters and palindromic sequences will yield more information of the prevalence of bidirectionality in promoters. To view the sequences we plan to test in this construct, please visit our parts page.