Team:Penn State/Bidirectional Promoters

From 2012.igem.org

(Difference between revisions)
 
(2 intermediate revisions not shown)
Line 52: Line 52:
border-bottom-color: #505050;
border-bottom-color: #505050;
}
}
 +
a:visited {color: #666}
a:hover {
a:hover {
Line 143: Line 144:
margin: 5px 0 15px;
margin: 5px 0 15px;
padding: 0 0 5px 0;
padding: 0 0 5px 0;
-
background-image: url(http://2012.igem.org/wiki/images/5/55/IndexNav1.jpg);
+
background-image: url(http://2012.igem.org/wiki/images/a/aa/Nav3.jpg);
background-repeat: no-repeat;
background-repeat: no-repeat;
background-position: left bottom;
background-position: left bottom;
Line 158: Line 159:
}
}
-
#desc h2 {
+
#desc h3 {
color: #fff;
color: #fff;
padding: 15px 15px 0;
padding: 15px 15px 0;
Line 295: Line 296:
}
}
-
#logoiGEM {
 
-
height: 114px;
 
-
width: 280px;
 
-
float: right;
 
-
background-image: url(http://2012.igem.org/wiki/images/4/4b/Sweatshirt_Logo.jpg);
 
-
padding-bottom: 15px;
 
-
background-repeat: no-repeat;
 
-
}
 
-
 
-
#logoPSU {
 
-
height: 54px;
 
-
width: 116px;
 
-
background-image: url(http://2012.igem.org/wiki/images/f/f9/PsuLogo.jpeg);
 
-
}
 
/*Tabbed Panels*/
/*Tabbed Panels*/
#maintabs {
#maintabs {
-
clear:both;
+
clear:both; margin-top: 15px;
}
}
Line 389: Line 376:
text-decoration:none;
text-decoration:none;
}
}
-
 
+
#logoiGEM {
 +
height: 56px;
 +
width: 140px;
 +
float: right;
 +
background-image: url(http://2012.igem.org/wiki/images/e/e9/Sweatshirt_Logofin.jpg);
 +
margin-bottom: 15px;
 +
background-repeat: no-repeat;
 +
}
 +
#psuLogo {
 +
        height: 65px;
 +
        width: 116;
 +
        float: right;
 +
        padding-top: 11px;
 +
}
</style>
</style>
</head>
</head>
-
<body>
 
 +
<body>
<div id="container">
<div id="container">
   <div id="logoiGEM"></div>
   <div id="logoiGEM"></div>
-
   <div id="logoPSU"></div>
+
   <div id="psuLogo"><img src="http://2012.igem.org/wiki/images/f/f9/PsuLogo.jpeg"></div>
   <div id="navitabs">
   <div id="navitabs">
     <h2 class="hide">Sample navigation menu:</h2>
     <h2 class="hide">Sample navigation menu:</h2>
Line 404: Line 404:
     <span class="hide"> | </span> <a class="activenavitab" href="http://2012.igem.org/Team:Penn_State/Project">Projects</a>
     <span class="hide"> | </span> <a class="activenavitab" href="http://2012.igem.org/Team:Penn_State/Project">Projects</a>
     <span class="hide"> | </span> <a class="navitab" href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a>
     <span class="hide"> | </span> <a class="navitab" href="http://2012.igem.org/Team:Penn_State/Parts">Parts</a>
-
     <span class="hide"> | </span> <a class="navitab" href="http://2012.igem.org/Team:Penn_State/Modeling">Modeling</a>
+
     <span class="hide"> | </span> <a class="navitab" href="http://2012.igem.org/Team:Penn_State/Modeling">Main Results</a>
     <span class="hide"> | </span> <a class="navitab" href="http://2012.igem.org/Team:Penn_State/Notebook">Notebook</a>
     <span class="hide"> | </span> <a class="navitab" href="http://2012.igem.org/Team:Penn_State/Notebook">Notebook</a>
     <span class="hide"> | </span> <a class="navitab" href="http://2012.igem.org/Team:Penn_State/Other">Other</a>
     <span class="hide"> | </span> <a class="navitab" href="http://2012.igem.org/Team:Penn_State/Other">Other</a>
Line 410: Line 410:
      
      
   <div id="desc">
   <div id="desc">
-
     <h2>Bidirectional Promoters</h2>
+
     <h3>Bidirectional Promoters</h3>
     <p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce  
     <p>Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce  
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote  
the expected proteins. Some bidirectional promoters are known to exist, but which way they promote  
Line 419: Line 419:
   <div id="main">
   <div id="main">
     <h2>Bidirectional Promoters</h2>
     <h2>Bidirectional Promoters</h2>
-
 
+
<iframe width="560" height="315" src="http://www.youtube.com/embed/s8eE07qU9yQ" frameborder="0" allowfullscreen></iframe>
     <div id="maintabs">
     <div id="maintabs">
     <h2 class="hide">Sample navigation menu:</h2>
     <h2 class="hide">Sample navigation menu:</h2>

Latest revision as of 03:05, 27 October 2012

Bidirectional Promoters Overview

Bidirectional Promoters

Scientists are frequently confounded by wayward promoters; that is, promoters which do not produce the expected proteins. Some bidirectional promoters are known to exist, but which way they promote and the degree of expression has not been quantified. This project will test the directionality of several BioBrick promoters to answer these questions.

Bidirectional Promoters

Sample navigation menu:

Overview | Design | Results

Background

Before we can make a protein, we need an mRNA to carry the information about the order of the amino acids. But before we can make mRNA we need to know where the genes are in the DNA of a cell. Before RNA polymerase can make a copy it needs to bind to the DNA. This is assisted by a variety of factors, other proteins, that look for a specific sequence in the DNA. This sequence is called a promoter because it promotes the transcription of the DNA into RNA by the RNA polymerase. These sequences are generally upstream, or ahead, of a gene's coding sequence.

The Problem

Not all promoters cause RNA polymerase to transcribe downstream in the expected "forward direction". Some promoters can cause RNA polymerase to go in the opposite direction from what is expected, or go in both directions. This is what we are trying to find out; do different promoters go in different directions, and what is the directional preference of different promoters.