Team:Osaka/week7

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Revision as of 07:57, 14 September 2012 by Toty (Talk | contribs)


September 9 (Sun)

  1. Miniprep of yesterday's culture(150,151,152,159,160)
  2. Restriction digests of minipreppped parts
  3. Electrophoresis
  4. Tolerance assay of 144,147(MMC)
  5. To liquid culture(144,145,160)


September 10 (Mon)

  1. Miniprep of yesterday's culture(160,1-9N)
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Preparation of LB agar plates(Amp)
  5. Ligation
    • 160(K):157(upstream)+158(downstream)+1-5A(vector)
  6. Transformation of ligation products
  7. To liquid culture(146,148)
  8. Tolerance assay of 144,145(MMC)


September 11 (Tue)

  1. Restriction digests(157,158,159,160,1-9N)
  2. Gel electrophoresis of digests
  3. Ligation
    • 160(K):157(upstream)+158(downstream)+1-5A(vector)
  4. Transformation of ligation products
  5. Tolerance assay of 146,148(MMC)


September 12 (Wed)

  1. Miniprep of yesterday's culture:160
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Tolerance assay
  5. Ligation
    • 161(C):1-9N(upstream)+160(downstream)+1-3A(vector)
    • 162(C):159(upstream)+160(downstream)+1-3A(vector)

September 13 (Thu)

  1. Colony check
    • 161:Transformed cells didnot grow
  2. Sequencing(157,158,159,160)
  3. Tolerance assay of 146,148
  4. Transformation of ligation products:161
  5. To liquid culture(144~148,162)


September 14 (Fri)

  1. Miniprep of yesterday's culture:162
  2. Restriction digests of minipreppped parts
  3. Tolerance assay (Preliminary test of hydrogen peroxide)
  4. Gel electrophoresis of digests
  5. To liquid culture(161)

September 15 (Sat)

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