Team:Osaka/week4
From 2012.igem.org
(Difference between revisions)
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<a href="https://2012.igem.org/Team:University_College_London/gemFM"> #gemFM </a> | <a href="https://2012.igem.org/Team:University_College_London/gemFM"> #gemFM </a> | ||
</html> | </html> | ||
+ | # Transfer to liquid culture: 155,156 | ||
- | |||
+ | ==August 25 (Sat)== | ||
+ | # Miniprep: 155,156 | ||
+ | # Restriction digests | ||
+ | # Electrophoresis:155,156,1-1G,1-3A,1-5A,1-7A | ||
+ | #*1-1G,1-3A,1-5A,1-7A → decompositioned | ||
+ | # Transformaton of ligation products into DH5α <i>E.coli</i>:1-1G,5-23O,1-5A,1-7A | ||
[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] |
Revision as of 07:07, 26 August 2012
August 19 (Sun)
- Colony check
- Transformed cells didn't grow on plates
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 20 (Mon)
- Colony check
- Transformed cells didn't grow on plates
- Transformation of plasmid DNA into DH5α E.coli
- test the efficiency of competent cells
August 21 (Tue)
- Colony check
- successfully transformed
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 22 (Wed)
- Transfer to liquid culture
- turned red → retrial
August 23 (Thu)
- Restriction digest
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 24 (Fri)
We had an interview about our project. #gemFM
- Transfer to liquid culture: 155,156
August 25 (Sat)
- Miniprep: 155,156
- Restriction digests
- Electrophoresis:155,156,1-1G,1-3A,1-5A,1-7A
- 1-1G,1-3A,1-5A,1-7A → decompositioned
- Transformaton of ligation products into DH5α E.coli:1-1G,5-23O,1-5A,1-7A