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Team:Osaka/week4
From 2012.igem.org
(Difference between revisions)
| Line 21: | Line 21: | ||
==August 22 (Wed)== | ==August 22 (Wed)== | ||
# Transfer to liquid culture | # Transfer to liquid culture | ||
| - | #* turned red | + | #* turned red → retrial |
==August 23 (Thu)== | ==August 23 (Thu)== | ||
| + | # Restriction digest | ||
| + | # Ligation:155,156 | ||
| + | # Transformaton of ligation products into DH5α <i>E.coli</i>:155,156 | ||
| + | |||
Revision as of 06:52, 26 August 2012
August 19 (Sun)
- Colony check
- Transformed cells didn't grow on plates
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 20 (Mon)
- Colony check
- Transformed cells didn't grow on plates
- Transformation of plasmid DNA into DH5α E.coli
- test the efficiency of competent cells
August 21 (Tue)
- Colony check
- successfully transformed
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 22 (Wed)
- Transfer to liquid culture
- turned red → retrial
August 23 (Thu)
- Restriction digest
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 24 (Fri)
We had an interview about our project. #gemFM
