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Team:Osaka/week4
From 2012.igem.org
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__NOTOC__ | __NOTOC__ | ||
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==August 19 (Sun)== | ==August 19 (Sun)== | ||
| + | # Colony check | ||
| + | #* Transformed cells didn't grow on plates | ||
# Ligation:155,156 | # Ligation:155,156 | ||
# Transformaton of ligation products into DH5α <i>E.coli</i>:155,156 | # Transformaton of ligation products into DH5α <i>E.coli</i>:155,156 | ||
==August 20 (Mon)== | ==August 20 (Mon)== | ||
| - | + | # Colony check | |
| + | #* Transformed cells didn't grow on plates | ||
| + | # Transformation of plasmid DNA into DH5α <i>E.coli</i> | ||
| + | #* test the efficiency of competent cells | ||
==August 21 (Tue)== | ==August 21 (Tue)== | ||
| - | + | # Colony check | |
| + | #* successfully transformed | ||
| + | # Ligation:155,156 | ||
| + | # Transformaton of ligation products into DH5α <i>E.coli</i>:155,156 | ||
==August 22 (Wed)== | ==August 22 (Wed)== | ||
| - | + | # Transfer to liquid culture | |
| + | #* turned red → retrial | ||
==August 23 (Thu)== | ==August 23 (Thu)== | ||
| + | # Restriction digest | ||
| + | # Ligation:155,156 | ||
| + | # Transformaton of ligation products into DH5α <i>E.coli</i>:155,156 | ||
| + | |||
==August 24 (Fri)== | ==August 24 (Fri)== | ||
| + | We had an interview about our project. | ||
| + | <html> | ||
| + | <a href="https://2012.igem.org/Team:University_College_London/gemFM"> #gemFM </a> | ||
| + | </html> | ||
| + | # Transfer to liquid culture: 155,156 | ||
==August 25 (Sat)== | ==August 25 (Sat)== | ||
| - | + | # Miniprep: 155,156 | |
| + | # Restriction digests | ||
| + | # Electrophoresis:155,156,1-1G,1-3A,1-5A,1-7A | ||
| + | #*155,156 × | ||
| + | # Transformaton of ligation products into DH5α <i>E.coli</i>:1-1G,5-23O,1-5A,1-7A | ||
[[Team:Osaka/Notebook|Back to Notebook]] | [[Team:Osaka/Notebook|Back to Notebook]] | ||
Latest revision as of 01:31, 14 September 2012
August 19 (Sun)
- Colony check
- Transformed cells didn't grow on plates
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 20 (Mon)
- Colony check
- Transformed cells didn't grow on plates
- Transformation of plasmid DNA into DH5α E.coli
- test the efficiency of competent cells
August 21 (Tue)
- Colony check
- successfully transformed
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 22 (Wed)
- Transfer to liquid culture
- turned red → retrial
August 23 (Thu)
- Restriction digest
- Ligation:155,156
- Transformaton of ligation products into DH5α E.coli:155,156
August 24 (Fri)
We had an interview about our project. #gemFM
- Transfer to liquid culture: 155,156
August 25 (Sat)
- Miniprep: 155,156
- Restriction digests
- Electrophoresis:155,156,1-1G,1-3A,1-5A,1-7A
- 155,156 ×
- Transformaton of ligation products into DH5α E.coli:1-1G,5-23O,1-5A,1-7A
