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Team:Osaka/week1
From 2012.igem.org
(Difference between revisions)
| Line 3: | Line 3: | ||
==August 1 (Wed)== | ==August 1 (Wed)== | ||
# Preparation of LB agar plates (Amp) | # Preparation of LB agar plates (Amp) | ||
| - | # Restriction digests of parts | + | # Restriction digests of parts (See Table 1) |
| + | {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5" | ||
| + | |+Table 1 | ||
| + | !ID!!Part Name!!Resistance!!Description | ||
| + | |- | ||
| + | |1-9N||<bbpart>BBa_J22106</bbpart>||A|| rec A (SOS) Promoter | ||
| + | |} | ||
| + | |||
| + | |||
# Ligation | # Ligation | ||
#* 143(A): 124+1-1G(vector) | #* 143(A): 124+1-1G(vector) | ||
Revision as of 04:44, 17 August 2012
August 1 (Wed)
- Preparation of LB agar plates (Amp)
- Restriction digests of parts (See Table 1)
| ID | Part Name | Resistance | Description |
|---|---|---|---|
| 1-9N | <bbpart>BBa_J22106</bbpart> | A | rec A (SOS) Promoter |
- Ligation
- 143(A): 124+1-1G(vector)
- 144(A): 125+1-1G(vector)
- 145(A): 126+1-1G(vector)
- 146(A): 127+1-1G(vector)
- 147(A): 128+1-1G(vector)
- 148(A): 129+1-1G(vector)
- 149(A): 138+1-1G(vector)
- 150(A): 139+1-1G(vector)
- 151(A): 140+1-1G(vector)
- 152(A): 141+1-1G(vector)
- Transformation of ligation products
