Team:Osaka/week1

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Latest revision as of 01:33, 14 September 2012

August 1 (Wed)

Preparation of LB agar plates (Amp)
Restriction digests of parts (See Table 1)
Ligation
- 143(A): 124+1-1G(vector)
- 144(A): 125+1-1G(vector)
- 145(A): 126+1-1G(vector)
- 146(A): 127+1-1G(vector)
- 147(A): 128+1-1G(vector)
- 148(A): 129+1-1G(vector)
- 149(A): 138+1-1G(vector)
- 150(A): 139+1-1G(vector)
- 151(A): 140+1-1G(vector)
- 152(A): 141+1-1G(vector)
Transformation of ligation products into DH5α E.coli

Table 1
number	Part Name	Resistance	Description
124	<bbpart>BBa_K602015</bbpart>	C	D. radiodurans PprI+PprA expression device
125	<bbpart>BBa_K602016</bbpart>	C	D. radiodurans PprI+RecA expression device
126	<bbpart>BBa_K602017</bbpart>	C	D. radiodurans PprA+RecA expression device
127	<bbpart>BBa_K602018</bbpart>	C	D. radiodurans PprI+PprM expression device
128	<bbpart>BBa_K602019</bbpart>	C	D. radiodurans PprA+PprM expression device
129	<bbpart>BBa_K602020</bbpart>	C	D. radiodurans PprM+RecA expression device
138	<bbpart>BBa_K602005</bbpart>	C	D. radiodurans PprI expression device
139	<bbpart>BBa_K602006</bbpart>	C	D. radiodurans PprA expression device
140	<bbpart>BBa_K602007</bbpart>	C	D. radiodurans PprM expression device
141	<bbpart>BBa_K602008</bbpart>	C	D. radiodurans RecA expression device

August 2 (Thu)

Transfer to liquid culture:143~152
- Some parts didn't successfully transformed

August 3 (Fri)

Miniprep of yesterday's culture
Restriction digests of minipreppped parts
Gel electrophoresis of digests
Transformation of ligation products into DH5α E.coli again:147,148,150

August 4 (Sat)

Transfer to liquid culture
Restriction digests of 1-1G<vector> with SpeI,XbaI
Ligation
Transformation of ligation products into DH5α E.coli
Transformation of plasmid DNA into Rosetta E.coli:143~146,149,152

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@@ Line 1: / Line 1: @@
-{{Osaka}}
+{{Osaka2}}
 __NOTOC__
 ==August 1 (Wed)==
 # Preparation of LB agar plates (Amp)
 # Restriction digests of parts (See Table 1)
+# Ligation
+#* 143(A): 124+1-1G(vector)
+#* 144(A): 125+1-1G(vector)
+#* 145(A): 126+1-1G(vector)
+#* 146(A): 127+1-1G(vector)
+#* 147(A): 128+1-1G(vector)
+#* 148(A): 129+1-1G(vector)
+#* 149(A): 138+1-1G(vector)
+#* 150(A): 139+1-1G(vector)
+#* 151(A): 140+1-1G(vector)
+#* 152(A): 141+1-1G(vector)
+# Transformation of ligation products into DH5α <i>E.coli</i>
 {|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 |+Table 1
@@ Line 28: / Line 42: @@
 |141||<bbpart>BBa_K602008</bbpart>||C|| <i>D. radiodurans</i> RecA expression device
 |}
-# Ligation
-#* 143(A): 124+1-1G(vector)
-#* 144(A): 125+1-1G(vector)
-#* 145(A): 126+1-1G(vector)
-#* 146(A): 127+1-1G(vector)
-#* 147(A): 128+1-1G(vector)
-#* 148(A): 129+1-1G(vector)
-#* 149(A): 138+1-1G(vector)
-#* 150(A): 139+1-1G(vector)
-#* 151(A): 140+1-1G(vector)
-#* 152(A): 141+1-1G(vector)
-# Transformation of ligation products
 ==August 2 (Thu)  ==
+# Transfer to liquid culture:143~152
+#* Some parts didn't successfully transformed
 ==August 3 (Fri)  ==
+# Miniprep of yesterday's culture
+# Restriction digests of minipreppped parts
+# Gel electrophoresis of digests
+# Transformation of ligation products into DH5α <i>E.coli</i> again:147,148,150
+==August 4 (Sat)  ==
-==August 4 (Fri)  ==
+# Transfer to liquid culture
+# Restriction digests of 1-1G<vector> with SpeI,XbaI
+# Ligation
+# Transformation of ligation products into DH5α <i>E.coli</i>
+# Transformation of plasmid DNA into Rosetta <i>E.coli</i>:143~146,149,152
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