Team:Osaka/week1

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{{Osaka}}
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__NOTOC__
__NOTOC__
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==August 1 (Wed)==
==August 1 (Wed)==
# Preparation of LB agar plates (Amp)
# Preparation of LB agar plates (Amp)
# Restriction digests of parts (See Table 1)
# Restriction digests of parts (See Table 1)
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 
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|+Table 1
 
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!number!!Part Name!!Resistance!!Description
 
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|-
 
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|124||<bbpart>BBa_K602015</bbpart>||C|| <i>D. radiodurans<i> PprI+PprA expression device
 
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|-
 
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|125||<bbpart>BBa_K602016</bbpart>||C|| <i>D. radiodurans<i> PprI+RecA expression device
 
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|-
 
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|126||<bbpart>BBa_K602017</bbpart>||C|| <i>D. radiodurans<i> PprA+RecA expression device
 
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|-
 
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|127||<bbpart>BBa_K602018</bbpart>||C|| <i>D. radiodurans<i> PprI+PprM expression device
 
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|-
 
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|128||<bbpart>BBa_K602019</bbpart>||C|| <i>D. radiodurans<i> PprA+PprM expression device
 
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|-
 
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|129||<bbpart>BBa_K602020</bbpart>||C|| <i>D. radiodurans<i> PprM+RecA expression device
 
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|}
 
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# Ligation
# Ligation
#* 143(A): 124+1-1G(vector)
#* 143(A): 124+1-1G(vector)
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#* 151(A): 140+1-1G(vector)
#* 151(A): 140+1-1G(vector)
#* 152(A): 141+1-1G(vector)
#* 152(A): 141+1-1G(vector)
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# Transformation of ligation products
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# Transformation of ligation products into DH5α <i>E.coli</i>
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==August 2 (Thu)  ==
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{|style="border-collapse: separate; border-spacing: 0; border-width: 1px; border-style: solid; border-color: #000; padding:5px;" cellpadding="5"
 +
|+Table 1
 +
!number!!Part Name!!Resistance!!Description
 +
|-
 +
|124||<bbpart>BBa_K602015</bbpart>||C|| <i>D. radiodurans</i> PprI+PprA expression device
 +
|-
 +
|125||<bbpart>BBa_K602016</bbpart>||C|| <i>D. radiodurans</i> PprI+RecA expression device
 +
|-
 +
|126||<bbpart>BBa_K602017</bbpart>||C|| <i>D. radiodurans</i> PprA+RecA expression device
 +
|-
 +
|127||<bbpart>BBa_K602018</bbpart>||C|| <i>D. radiodurans</i> PprI+PprM expression device
 +
|-
 +
|128||<bbpart>BBa_K602019</bbpart>||C|| <i>D. radiodurans</i> PprA+PprM expression device
 +
|-
 +
|129||<bbpart>BBa_K602020</bbpart>||C|| <i>D. radiodurans</i> PprM+RecA expression device
 +
|-
 +
|138||<bbpart>BBa_K602005</bbpart>||C|| <i>D. radiodurans</i> PprI expression device
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|-
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|139||<bbpart>BBa_K602006</bbpart>||C|| <i>D. radiodurans</i> PprA expression device
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|-
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|140||<bbpart>BBa_K602007</bbpart>||C|| <i>D. radiodurans</i> PprM expression device
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|-
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|141||<bbpart>BBa_K602008</bbpart>||C|| <i>D. radiodurans</i> RecA expression device
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|}
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==August 2 (Thu)  ==
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# Transfer to liquid culture:143~152
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#* Some parts didn't successfully transformed
==August 3 (Fri)  ==
==August 3 (Fri)  ==
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# Miniprep of yesterday's culture
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# Restriction digests of minipreppped parts
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# Gel electrophoresis of digests
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# Transformation of ligation products into DH5α <i>E.coli</i> again:147,148,150
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==August 4 (Sat)  ==
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==August 4 (Fri)  ==
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# Transfer to liquid culture
-
 
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# Restriction digests of 1-1G<vector> with SpeI,XbaI
 +
# Ligation
 +
# Transformation of ligation products into DH5α <i>E.coli</i>
 +
# Transformation of plasmid DNA into Rosetta <i>E.coli</i>:143~146,149,152
[[Team:Osaka/Notebook|Back to Notebook]]
[[Team:Osaka/Notebook|Back to Notebook]]

Latest revision as of 01:33, 14 September 2012


August 1 (Wed)

  1. Preparation of LB agar plates (Amp)
  2. Restriction digests of parts (See Table 1)
  3. Ligation
    • 143(A): 124+1-1G(vector)
    • 144(A): 125+1-1G(vector)
    • 145(A): 126+1-1G(vector)
    • 146(A): 127+1-1G(vector)
    • 147(A): 128+1-1G(vector)
    • 148(A): 129+1-1G(vector)
    • 149(A): 138+1-1G(vector)
    • 150(A): 139+1-1G(vector)
    • 151(A): 140+1-1G(vector)
    • 152(A): 141+1-1G(vector)
  4. Transformation of ligation products into DH5α E.coli
Table 1
numberPart NameResistanceDescription
124<bbpart>BBa_K602015</bbpart>C D. radiodurans PprI+PprA expression device
125<bbpart>BBa_K602016</bbpart>C D. radiodurans PprI+RecA expression device
126<bbpart>BBa_K602017</bbpart>C D. radiodurans PprA+RecA expression device
127<bbpart>BBa_K602018</bbpart>C D. radiodurans PprI+PprM expression device
128<bbpart>BBa_K602019</bbpart>C D. radiodurans PprA+PprM expression device
129<bbpart>BBa_K602020</bbpart>C D. radiodurans PprM+RecA expression device
138<bbpart>BBa_K602005</bbpart>C D. radiodurans PprI expression device
139<bbpart>BBa_K602006</bbpart>C D. radiodurans PprA expression device
140<bbpart>BBa_K602007</bbpart>C D. radiodurans PprM expression device
141<bbpart>BBa_K602008</bbpart>C D. radiodurans RecA expression device

August 2 (Thu)

  1. Transfer to liquid culture:143~152
    • Some parts didn't successfully transformed

August 3 (Fri)

  1. Miniprep of yesterday's culture
  2. Restriction digests of minipreppped parts
  3. Gel electrophoresis of digests
  4. Transformation of ligation products into DH5α E.coli again:147,148,150

August 4 (Sat)

  1. Transfer to liquid culture
  2. Restriction digests of 1-1G<vector> with SpeI,XbaI
  3. Ligation
  4. Transformation of ligation products into DH5α E.coli
  5. Transformation of plasmid DNA into Rosetta E.coli:143~146,149,152

Back to Notebook

Retrieved from "http://2012.igem.org/Team:Osaka/week1"