Team:Michigan/Notebook/Protocols

From 2012.igem.org

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Protocols
Protocols
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<h2>General</h2>
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Plate Production</html>[https://static.igem.org/mediawiki/2012/0/08/MichPlate.pdf]<html>
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<h2>Cloning</h2>
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Removing BioBricks from the Registry</html>[https://static.igem.org/mediawiki/2012/5/50/MichRem.pdf]<html>
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!Protocols
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; Heat Shock
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<!-- This page will be updated to display previous versions in a dropdown box (HTML select embedded in a form) soon. In the meantime, upload protocols, or link to previous wiki or external websites.-->
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[[File:Quick heat shock protocol v1.0.pdf]]
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Production of Competent Cells</html>[https://static.igem.org/mediawiki/2012/6/61/MichComp.pdf]<html>
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[[File:Heat shock protocol with SOC rescue v1.1.pdf]]
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Heat Shock Transformation</html>[https://static.igem.org/mediawiki/2012/0/0e/MichHeat.pdf]<html>
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[[File:Heat shock protocol with SOC rescue v1.0.pdf]]
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;Gel electrophoresis
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[[File:Gel electrophoresis v1.0.pdf]]
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DNA Purification: PCR</html>[https://static.igem.org/mediawiki/2012/0/04/MichPur2.pdf]<html>
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DNA Purification: MiniPrep</html>[https://static.igem.org/mediawiki/2012/1/19/MichPur1.pdf]<html>
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Restriction Enzyme Digest</html>[https://static.igem.org/mediawiki/2012/0/0f/MichRestrict.pdf]<html>
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Ligation</html>[https://static.igem.org/mediawiki/2012/1/10/MichLig.pdf]<html>
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PCR (Phusion)</html>[https://static.igem.org/mediawiki/2012/9/94/MichPCR.pdf]<html>
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Colony PCR</html>[https://static.igem.org/mediawiki/2012/9/96/MichCol.pdf]<html>
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Gel Electrophoresis</html>[https://static.igem.org/mediawiki/2012/1/12/MichElectro.pdf]<html>
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<h2>Characterization</h2>
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Transmission Electron Microscopy Protocol</html>[https://static.igem.org/mediawiki/2012/0/0a/MichTEM.pdf]<html>
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FimE Asymmetrical Digest Assay Protocol</html>[https://static.igem.org/mediawiki/2012/a/aa/MichAsm.pdf]<html>
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Latest revision as of 02:26, 4 October 2012

Xayona Website Template

Protocols

General

Plate Production[1]

Cloning

Removing BioBricks from the Registry[2]

Production of Competent Cells[3]

Heat Shock Transformation[4]

DNA Purification: PCR[5] DNA Purification: MiniPrep[6]

Restriction Enzyme Digest[7]

Ligation[8]

PCR (Phusion)[9]

Colony PCR[10]

Gel Electrophoresis[11]

Characterization


Transmission Electron Microscopy Protocol[12]

FimE Asymmetrical Digest Assay Protocol[13]

Retrieved from "http://2012.igem.org/Team:Michigan/Notebook/Protocols"