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Team:Michigan/Notebook
From 2012.igem.org
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| - | <h2>Modify HbiF into a Biobrick compatible format and | + | <h2>Modify HbiF into a Biobrick compatible format </h2> |
| + | <p>HbiF and LVA degradation tag were ordered from IDT with the following specifications.</p> | ||
| + | |||
| + | <p> <span style="background-color:#E0771B;font-family:Courier, monospace;">EcoRI</span></p> | ||
| + | <p> <span style="background-color:#E0B91B;font-family:Courier, monospace;">XbaI</span></p> | ||
| + | <p> <span style="background-color:#C8E0DB;font-family:Courier, monospace;">SpeI</span></p> | ||
| + | <p> <span style="background-color:#E0771B;font-family:Courier, monospace;">PstI</span></p> | ||
| + | <p> <span style="background-color:#E0771B;font-family:Courier, monospace;">AgeI</span></p> | ||
| + | <p> <span style="background-color:#E0771B;font-family:Courier, monospace;">NgoMIV</span></p> | ||
| + | |||
| + | <p><br/>HbiF possesses an N-terminus in the (truncated) RFC10 format :<br/></p> | ||
| + | |||
| + | <p style="font-family:Courier, monospace;"><span style="background-color:#E0771B">GAATTC</span> GCGGCCGC T <span style="background-color:#E0B91B;">TCTAG</span> [ATG]</p> | ||
| + | |||
| + | <h2>Prepare FimE for protein expression</h2> | ||
<iframe width=100% height=500; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&embedded=true"></iframe> | <iframe width=100% height=500; src="https://docs.google.com/document/pub?id=1bDaN8iqQjBHllPLosoQUs9Z9MZT4Q3pjOG3M_9UMEho&embedded=true"></iframe> | ||
<h2>Team Reports</h2> | <h2>Team Reports</h2> | ||
Revision as of 20:26, 2 July 2012
Team:Michigan/Notebook/Protocols
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Modify HbiF into a Biobrick compatible format
HbiF and LVA degradation tag were ordered from IDT with the following specifications.
EcoRI
XbaI
SpeI
PstI
AgeI
NgoMIV
HbiF possesses an N-terminus in the (truncated) RFC10 format :
GAATTC GCGGCCGC T TCTAG [ATG]
Prepare FimE for protein expression
Team Reports
