Team:METU/CODH

From 2012.igem.org

(Difference between revisions)
Line 1: Line 1:
<html lang="en-US" xmlns="http://www.w3.org/1999/xhtml" dir="ltr">
<html lang="en-US" xmlns="http://www.w3.org/1999/xhtml" dir="ltr">
-
<head>
+
    <head>
-
<meta http-equiv="Content-type" content="text/html; charset=utf-8" />
+
        <meta http-equiv="Content-type" content="text/html; charset=utf-8" />
 +
 
-
 
 
         <link rel="shortcut icon" href="http://simsekburak.com/hasan/css/images/favicon.ico" />
         <link rel="shortcut icon" href="http://simsekburak.com/hasan/css/images/favicon.ico" />
-
<link rel="stylesheet" href="http://simsekburak.com/hasan/css/style.css" type="text/css" media="all" />
+
        <link rel="stylesheet" href="http://simsekburak.com/hasan/css/style.css" type="text/css" media="all" />
-
<!--[if IE 6]>
+
        <!--[if IE 6]>
-
<link rel="stylesheet" href="css/ie6.css" type="text/css" media="all" />
+
                <link rel="stylesheet" href="css/ie6.css" type="text/css" media="all" />
-
<script src="http://simsekburak.com/hasan/js/png-fix.js" type="text/javascript"></script>
+
                <script src="http://simsekburak.com/hasan/js/png-fix.js" type="text/javascript"></script>
-
<![endif]-->
+
        <![endif]-->
-
<script src="http://simsekburak.com/hasan/js/jquery-1.4.2.js" type="text/javascript"></script>
+
        <script src="http://simsekburak.com/hasan/js/jquery-1.4.2.js" type="text/javascript"></script>
-
<script src="http://simsekburak.com/hasan/js/jquery.jcarousel.js" type="text/javascript"></script>
+
        <script src="http://simsekburak.com/hasan/js/jquery.jcarousel.js" type="text/javascript"></script>
-
<script src="http://simsekburak.com/hasan/js/js-func.js" type="text/javascript"></script>
+
        <script src="http://simsekburak.com/hasan/js/js-func.js" type="text/javascript"></script>
-
 
+
-
<style>
+
-
h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{
+
-
  display:none;
+
-
}
+
-
 
+
-
#top-section{
+
-
height: 20px !important;
+
-
border: none !important;
+
-
}
+
-
div#content{
+
        <style>
-
padding: 0 !important;
+
            h1.firstHeading, div#p-logo, div#search-controls, div#contentSub, div#footer-box, div#catlinks{
-
background: transparent;
+
                display:none;
-
border: none !important;
+
            }
-
width: auto;
+
-
}
+
-
</style>
+
            #top-section{
 +
                height: 20px !important;
 +
                border: none !important;
 +
            }
 +
            div#content{
 +
                padding: 0 !important;
 +
                background: transparent;
 +
                border: none !important;
 +
                width: auto;
 +
            }
-
</head>
+
        </style>
-
<body>
+
-
<!-- Header -->
+
    </head>
 +
    <body>
 +
        <!-- Header -->
<div id="header">
<div id="header">
<div class="shell">
<div class="shell">
Line 52: Line 50:
    <li><a href="https://2012.igem.org/Team:METU/Team"><span>Team</span></a></li>
    <li><a href="https://2012.igem.org/Team:METU/Team"><span>Team</span></a></li>
-
    <li><a href="https://2012.igem.org/Team:METU/Project"><span>Project</span></a>
+
    <li><a href="#"><span>Project</span></a>
<div class="dd-holder">
<div class="dd-holder">
<div class="dd-t"></div>
<div class="dd-t"></div>
<div class="dd">
<div class="dd">
<ul>
<ul>
-
<li><a href="https://2012.igem.org/Team:METU/Project"><span>Overview</span></a></li>
+
<li><a href="https://2012.igem.org/Team:METU/ProjectOverview"><span>Overview</span></a></li>
<li><a href="https://2012.igem.org/Team:METU/CODH"><span>CODH</span></a></li>
<li><a href="https://2012.igem.org/Team:METU/CODH"><span>CODH</span></a></li>
<li><a href="https://2012.igem.org/Team:METU/KillSwitchOverview"><span>Kill-Switch</span></a></li>
<li><a href="https://2012.igem.org/Team:METU/KillSwitchOverview"><span>Kill-Switch</span></a></li>
<li><a href="https://2012.igem.org/Team:METU/CellLimiter"><span>Cell Limiter</span></a></li>
<li><a href="https://2012.igem.org/Team:METU/CellLimiter"><span>Cell Limiter</span></a></li>
-
<li><a href="https://2012.igem.org/Team:METU/datapage"><span>Data Page</span></a></li>
+
<li class="last"><a href="https://2012.igem.org/Team:METU/datapage"><span>Data Page</span></a></li>
</ul>
</ul>

Revision as of 17:28, 25 September 2012

CODH

 

Overview

The aim of the Project is to express CODH (Carbon monoxide dehydrogenase) enzyme found in different types of bacteria in cyanobacteria. This enzyme converts CO to CO2 using the reactions below:

CO2 + 2H+ + 2e- <-> CO + H2O

Once the CO2 binds, there is a subtle change in the shape of the enzyme that prohibits the CO, when released from the active site, from escaping into the cytoplasm. Being cut off, there is only one escape: move through the molecular tunnel in the enzyme to the A cluster situated by the exit. One electron comes from the reduced B cluster, and one electron cones from the reduce C cluster itself. This is a reversible reaction; it's conceivable that the toxic effects of CO can be somewhat neutralized by the cell by turning it into CO2. However, since CO is not common in the environment it is doubtful that the bacterium uses the reversed reaction to save itself.

How the System Works?

By the way, the name carbon monoxide dehydrogenase comes from the class of enzymes to which this activity belongs. You'll notice there is no hydrogen on CO2 to dehydrogenate, but if you reverse the reaction above, you can see what is dehydrogenated. CODH is important for maintaining current atmospheric conditions. Microbial metabolisms of CO maintains ambient CO at levels safe for other forms of life.