Team:KIT-Kyoto/Notebook-week3p
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Revision as of 10:21, 25 September 2012
September 6th
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September 11th
September 12thComposition
The digested DNA were applied to agarose gel electrophoresis. We isolated DNA from the gel by using QIA quick Gel Extraction Kit. Photo of Agarose gel. 1-1-48 and 2-2-6 Ligation DNA fragment carrying EGFP or LacZ was ligated into the BglII and SpeI digested pSB1C3-UAS DNA .for 1hour at 16℃. Ligation reactions are listed below.
We ligated DNA fragment carrying GAL4 and DNA fragments carrying HS promoter or Act5c promoter-enhancer to pSB1C3 DNA for 1hour at 16℃. Composition
1-1-49 and 2-2-7 Transformation of E. coli by Ligation products We did transformation of E. coli Xl-1 blue with each of ligation products. Finally, we spread them on the LB Chloramphenicol(+) plate and incubated for 16 hours at 37℃. |