Team:Exeter/lab book/proto

From 2012.igem.org

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     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/11" style="color:#1d1d1b"><u>Preparation of IDTE Buffer and IDT Re-Suspension</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/11" style="color:#1d1d1b"><u>Preparation of IDTE Buffer and IDT Re-Suspension</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/12" style="color:#1d1d1b"><u>3-Step PCR</u></a></li>
     <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/12" style="color:#1d1d1b"><u>3-Step PCR</u></a></li>
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    <li type="1" style="line-height:3"><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/13" style="color:#1d1d1b"><u>SDS-PAGE Protocol</u></a></li>
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Revision as of 16:51, 25 September 2012

Protocol Home

Protocols
  • Transformation Protocol from Invitrogen, for Top10 Competent Cells
  • Transferring Cultures to Liquid Medium
  • Mini Prep
  • New England BioLabs Biobrick Assembly Protocol
  • Annealing Oligomer Primers
  • Biobrick Extraction
  • Two-Step PCR
  • Bacterial Genomic DNA Extraction Using GenElute
  • PCR purification using QIAquick
  • Linearized Plasmid Backbone Digestion and Ligation Protocol
  • Preparation of IDTE Buffer and IDT Re-Suspension
  • 3-Step PCR
  • SDS-PAGE Protocol