Team:Exeter/lab book/gibs/wk6

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      <a href="https://2012.igem.org/Team:Exeter/lab_book/proto"; style="color:#57b947">Protocols</a>
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      &nbsp;|&nbsp;
       <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk1"; style="color:#57b947">Single Gene Plasmids and Enzyme Characterisation</a>
       <a href="https://2012.igem.org/Team:Exeter/lab_book/1gp/wk1"; style="color:#57b947">Single Gene Plasmids and Enzyme Characterisation</a>
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         <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk10"; style="color:#1d1d1b">10th - 14th September</a>
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         <a href="https://2012.igem.org/Team:Exeter/Results#usepoly"; style="color:#e30614" target="_blank"><font size="3"><b>Results: Part 1</b></font></a>
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         <a href="https://2012.igem.org/Team:Exeter/lab_book/gibs/wk11"; style="color:#1d1d1b">17th - 21st September</a>
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         <a href="https://2012.igem.org/Team:Exeter/Results/GvsB"; style="color:#e30614" target="_blank"><font size="3"><b>Results: Part 2</b></font></a>
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       <p><b><u>Operon Construction: 13th - 17th August 2012</u></b></p>
       <p><b><u>Operon Construction: 13th - 17th August 2012</u></b></p>
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     <!------------ENTER LAB BOOK HERE: PLEASE INCLUDE DATES WITHIN THE WEEK------------>  
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     <!<p><b>**Tuesday 14.8.12**</b></p><br><p>
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</p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/1" style="color:#57b947"><u>Transformation</u></a> of <i>wcfA</i>-term (BBa_K764010)</p><br>
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<p><b>**Wednesday 15.8.12**</b></p><br>
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<p><a href="https://2012.igem.org/Team:Exeter/lab_book/proto/2" style="color:#57b947"><u>Transferred <i>wcfA</i>-term colonies to LB(tet) medium</u></a></p><br>
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<p><b>**Thursday 16.8.12**</b></p><br>
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<p><i>wfcA</i>-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C</p>
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<p>Supernatant discarded</p>
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<p><a href="http://www.fermentas.com/templates/files/tiny_mce/coa_pdf/coa_k0502.pdf" style="color:#57B947" target="_blank"><u>Fermentas protocol</u></a></p>
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<p><b><i>Step 10</i></b> 45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation</p>
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<p>Nanodropped for following results: </p><p>
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(A) 329.7 ng/µl</p><p>
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(B) 50.0 ng/µl</p><p>
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(C) 206.6 ng/µl</p><br><p>
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Made streak plates from culture dregs</p>
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    <p><u>Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley</u> &nbsp;&nbsp;|&nbsp;&nbsp;
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    <a href="https://igem.org/Team.cgi?id=764" style="color:#57B947" target="_blank"><u>Contact Us</u></a>  &nbsp;&nbsp;|&nbsp;&nbsp;
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    <a href="https://2012.igem.org/Team:Exeter/site_map" style="color:#57B947"><u>Site Map</u></a></p>
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Latest revision as of 00:10, 27 September 2012

ExiGEM2012 Lab Book Gibs wk6

Operon Construction: 13th - 17th August 2012

 **Tuesday 14.8.12**


Transformation of wcfA-term (BBa_K764010)


**Wednesday 15.8.12**


Transferred wcfA-term colonies to LB(tet) medium


**Thursday 16.8.12**


wfcA-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C

Supernatant discarded

Fermentas protocol

Step 10 45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation

Nanodropped for following results:

(A) 329.7 ng/µl

(B) 50.0 ng/µl

(C) 206.6 ng/µl


Made streak plates from culture dregs

Website Designed and Built by: Ryan Edginton, James Lynch & Alex Clowsley   |   Contact Us   |   Site Map

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