Team:Exeter/lab book/gibs/wk6

From 2012.igem.org

(Difference between revisions)
Line 92: Line 92:
         -
         -
         </p>
         </p>
-
         <a href="https://2012.igem.org/Team:Exeter/Results/usefulpoly"; style="color:#e30614"><font size="3"><b>Results: Part 1</b></font></a>
+
         <a href="https://2012.igem.org/Team:Exeter/Results#usepoly"; style="color:#e30614"><font size="3"><b>Results: Part 1</b></font></a>
         <p>
         <p>
         -
         -

Revision as of 20:13, 26 September 2012

ExiGEM2012 Lab Book Gibs wk6

Operon Construction: 13th - 17th August 2012

**Tuesday 14.8.12**


Transformation of wcfA-term (BBa_K764010)


**Wednesday 15.8.12**


Transferred wcfA-term colonies to LB(tet) medium


**Thursday 16.8.12**


wfcA-term transformed cell broth tubes centrifuged 10 minutes, 3901 rcf, 4 °C

Supernatant discarded

Fermentas protocol

Step 10 45µl milli-Q water added in place of elution buffer, followed by another 5 µl after incubation and centrifugation

Nanodropped for following results:

(A) 329.7 ng/µl

(B) 50.0 ng/µl

(C) 206.6 ng/µl


Made streak plates from culture dregs