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Team:Evry/OrthogonalSyst
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<h2>Auxin reception device</h2> | <h2>Auxin reception device</h2> | ||
| - | <p>We envisaged 2 ways of design our auxin production device in tadpoles. To visualize the communication between different tissues or between E.coli and a tissue of the tadpole, we chose to work with GFP.</p> | + | <p>We envisaged 2 ways of design our auxin production device in tadpoles. To visualize the communication between different tissues or between E.coli and a tissue of the tadpole, we chose to work with GFP.Our orthogonal hormonal system <b>works with any proteins fused to AID signal</b> with any transcription factors.</p> |
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<li> <b>Auxin reception device 1 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a> coding for GFP-AID, and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812012">BBa_K812012</a> coding for OsTir1. | <li> <b>Auxin reception device 1 :</b> this device is composed of <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812010">BBa_K812010</a> coding for GFP-AID, and <a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812012">BBa_K812012</a> coding for OsTir1. | ||
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Revision as of 17:40, 23 September 2012
Orthogonal hormonal system
Our orthogonal hormonal system is based on the use of auxin, a plant hormone. To implement this system, we used two different types of device corresponding to the production and the reception of auxin in the tadpole.
Auxin production devices
We envisaged 3 ways of design our auxin production device in tadpoles. The devices 1 and 2 have been designed to be expressed in tadpole's embryos, while device 3 have been designed to be expressed in E.coli. In this last case, the aim is that the tadpoles eat bacteria expressing device 3.
- Auxin production device 1 : this device is composed of BBa_K812021, coding for IaaM, and BBa_K812120, coding for IaaH for auxin generator for the use in tadpole's embryos.
- Auxin production device 2 : this device is composed of BBa_K812014. It is ment for the co-expression of IaaH and IaaM genes in the same cell in tadpole.
- Auxin production device 3 : this device is composed of BBa_K515100, coding for IaaM and IaaH for auxin generator in E.coli.
Pathway
Auxin reception device
We envisaged 2 ways of design our auxin production device in tadpoles. To visualize the communication between different tissues or between E.coli and a tissue of the tadpole, we chose to work with GFP.Our orthogonal hormonal system works with any proteins fused to AID signal with any transcription factors.
- Auxin reception device 1 : this device is composed of BBa_K812010 coding for GFP-AID, and BBa_K812012 coding for OsTir1.
- Auxin reception device 2 : this device is composed of BBa_K812013 coding for GFP-AID and OsTir1 in the same cell.
References:
- 1. Nishimura, K., Fukagawa, T., Takisawa, H., Kakimoto, T. & Kanemaki, M. An auxin-based degron system for the rapid depletion of proteins in nonplant cells. Nature methods 6, 917-22 (2009).
