Team:Evry/Notebook/w15

From 2012.igem.org

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<h1>Weeks</h1>
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<h1>Week 15: 17th September - 23rd September</h1>
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<p> this week we have worked to characterize Promotors and Reporters in order to test their validity by injection in tadpoles :
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<ul>
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<li>biobricking promotors in Pcs2+ which are then to be characterized in tadpoles after cloning upstream to various reporters including mCFP, SfGFP, and citrine which are:
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            <ul><li>P Elastase</li>
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                    <li>Hsp70</li>
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                    <li>P Car A</li>
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                    <li> FLk1</li>
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                    <li>P CMV</li></ul>
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this work included standard PCR amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.</ul>
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<li>following that  cloning the reporters downstream to various promotors was done </li>
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<li> cloning og IAAh and IAAM in both Psb1C3 and Pcs2+  by standard biobrick portocol </li>
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<li> Golden gate assembly of IAAM-IAAH cassette in both Psb1C3 and Pcs2+ </li>
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<li>Injection in tadpoles for the parts we managed to clone and was validated correctly by sequencing </li>
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w1">1</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w2">2</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w3">3</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w4">4</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w5">5</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w6">6</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w7">7</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w8">8</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w9">9</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w10">10</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w11">11</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w12">12</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w13">13</a></td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w14">14</a></td>
 
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  <td>15</td>
 
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  <td><a href="https://2012.igem.org/Team:Evry/Notebook/w16">16</a></td>
 
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</table>
 
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<h1>Week 15: 17th September - 23rd September</h1>
 
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Latest revision as of 02:21, 27 September 2012

Weeks:

June July August September October November

Week 15: 17th September - 23rd September

this week we have worked to characterize Promotors and Reporters in order to test their validity by injection in tadpoles :

  • biobricking promotors in Pcs2+ which are then to be characterized in tadpoles after cloning upstream to various reporters including mCFP, SfGFP, and citrine which are:
    • P Elastase
    • Hsp70
    • P Car A
    • FLk1
    • P CMV
    this work included standard PCR amplification, purification , plasmid digestion, ligation of vector-insert, transformation, miniprepping, and sequencing.
  • following that cloning the reporters downstream to various promotors was done
  • cloning og IAAh and IAAM in both Psb1C3 and Pcs2+ by standard biobrick portocol
  • Golden gate assembly of IAAM-IAAH cassette in both Psb1C3 and Pcs2+
  • Injection in tadpoles for the parts we managed to clone and was validated correctly by sequencing

    • Retrieved from "http://2012.igem.org/Team:Evry/Notebook/w15"