Team:Evry/Notebook/w13

From 2012.igem.org

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<h2>Monday 3rd</h2>
<h2>Monday 3rd</h2>
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'''[[
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<u><h5>Construction about a new pCS2+ with RFP insert</u></h5>
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Construction about a new pCS2+ with RFP insert :]]'''
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-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI
 
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-Purification gel to get only RFP insert ( gel image )
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-Digestion of the miniprep about pCSB1C3 with RFP insert  by EcoRI and PstI <br>
 +
 
 +
 
 +
-Purification gel to get only RFP insert ( gel image )<br>
 +
 
 +
 
 +
-Extraction of the RFP insert <br>
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-Extraction of the RFP insert
 
-Ligation with pCS2+
-Ligation with pCS2+
 +
 +
<h5>Injection into tadpoles</h5>
 +
 +
Injection of plasmid pCS2+ with the CMV promoter and GFP-aid.
 +
This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work.
<h2>Tuesday 3rd</h2>
<h2>Tuesday 3rd</h2>
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'''[[
 
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'''[[Preparation of a new stock of electrocompetent cells T10]]'''
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 +
<u><h5>Preparation of a new stock of electrocompetent cells T10</u></h5>
 +
 
 +
<br>
 +
<br>
 +
 
 +
<u><h5>Construction about a new pCS2+ with RFP insert :</u></h5>
 +
<i>(Following)</i><br>
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Construction about a new pCS2+ with RFP insert :]]'''
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-Transformation by electroporation with T10 cells<br>
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''Following''
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-
-Transformation by electroporation with T10 cells
 
-Plates prepared
-Plates prepared

Latest revision as of 03:06, 27 September 2012

Weeks:

June July August September October November

Week 13: 3rd September - 9th September

Monday 3rd

Construction about a new pCS2+ with RFP insert
-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI
-Purification gel to get only RFP insert ( gel image )
-Extraction of the RFP insert
-Ligation with pCS2+
Injection into tadpoles
Injection of plasmid pCS2+ with the CMV promoter and GFP-aid. This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work.

Tuesday 3rd

Preparation of a new stock of electrocompetent cells T10


Construction about a new pCS2+ with RFP insert :
(Following)
-Transformation by electroporation with T10 cells
-Plates prepared