"
Team:Evry/Notebook/w13
From 2012.igem.org
(Difference between revisions)
(Created page with "{{:Team:Evry/template_v1}} <html> <h1>Weeks</h1> <table> <tr> <td><a href="https://2012.igem.org/Team:Evry/Notebook/w1">1</a></td> <td><a href="https://2012.igem.org/Team:Evr...") |
|||
| (7 intermediate revisions not shown) | |||
| Line 1: | Line 1: | ||
| - | {{:Team:Evry/ | + | {{:Team:Evry/template_notebook}} |
| + | |||
<html> | <html> | ||
| - | <h1> | + | <h1>Week 13: 3rd September - 9th September</h1> |
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | |||
| - | < | + | <h2>Monday 3rd</h2> |
| + | <u><h5>Construction about a new pCS2+ with RFP insert</u></h5> | ||
| + | |||
| + | |||
| + | -Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI <br> | ||
| + | |||
| + | |||
| + | -Purification gel to get only RFP insert ( gel image )<br> | ||
| + | |||
| + | |||
| + | -Extraction of the RFP insert <br> | ||
| + | |||
| + | |||
| + | -Ligation with pCS2+ | ||
| + | |||
| + | <h5>Injection into tadpoles</h5> | ||
| + | |||
| + | Injection of plasmid pCS2+ with the CMV promoter and GFP-aid. | ||
| + | This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work. | ||
| + | |||
| + | <h2>Tuesday 3rd</h2> | ||
| + | |||
| + | |||
| + | <u><h5>Preparation of a new stock of electrocompetent cells T10</u></h5> | ||
| + | |||
| + | <br> | ||
| + | <br> | ||
| + | |||
| + | <u><h5>Construction about a new pCS2+ with RFP insert :</u></h5> | ||
| + | <i>(Following)</i><br> | ||
| + | |||
| + | |||
| + | -Transformation by electroporation with T10 cells<br> | ||
| + | |||
| + | |||
| + | -Plates prepared | ||
| + | |||
</html> | </html> | ||
Latest revision as of 03:06, 27 September 2012
Weeks:
| June | July | August | September | October | November | ||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Week 13: 3rd September - 9th September
Monday 3rd
Construction about a new pCS2+ with RFP insert
-Digestion of the miniprep about pCSB1C3 with RFP insert by EcoRI and PstI-Purification gel to get only RFP insert ( gel image )
-Extraction of the RFP insert
-Ligation with pCS2+
Injection into tadpoles
Injection of plasmid pCS2+ with the CMV promoter and GFP-aid. This experiment was throughout the week. Taking pictures and take care of tadpole is a full-time work.Tuesday 3rd
Preparation of a new stock of electrocompetent cells T10
Construction about a new pCS2+ with RFP insert :
(Following)-Transformation by electroporation with T10 cells
-Plates prepared
