Team:Evry/Notebook/July/6

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Promoters & Reporters workgroup

Digestion

Miniprep 28/06/2012

masse DNA = 2000 ng Volume final = 30 uL

DNA
Concentration (ng.uL-1)
V DNA (uL)
V SpeI (uL)
V EcoRI (uL)
V XBA I (uL)
V Buffer Orange (uL)
V Buffer sup (uL)
V water (uL)
pCS2 +
362,7
5,51
0
1
1
3
0
19,49
CFP
240,1
8,33
1
1
0
0
3
16,67
GFP
209,1
9,56
1
1
0
0
3
15,44
RFP
142,8
14,01
1
1
0
0
3
10,99
YFP
196,0
10,20
1
1
0
0
3
14,80


Incubation 3h at 37 degrees Celsius

Gel Migration

Gel at 0,08%
V tae = 30 mL
m aga = 0,24 g
V bet = 3 uL

No Band for RFP (again)

Gel Extraction

see protocol on 05/07/2012

Type
Gel weight (g)
V QG (uL)
V iso (uL)
pCS2 +
0,37
1 110
370
YFP
0,17
510
170
GFP
0,23
690
230
CFP
0,19
570
190


DNA Concentration
Type
Concentration (ng.uL-1)
pCS2 +
16,7
GFP
8,5
CFP
9,1
YFP
5,6


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