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From 2012.igem.org

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Primers

<tdcgctgcagGAACTATAGTGAGTCGTATTACG </td><td>23 </td><td>51 </td><td>39 </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P8 </td><td>pCS2+BB-Rv </td><td>tgcatGAATTCGAATCGATGGGATCC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P9 </td><td>GFP-AID-BB-Fw </td><td>gaattcgcggccgcttctagagATGGCCACAACCATGGTG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P10 </td><td>GFP-AID-BB-Rv </td><td>tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P11 </td><td>p2 Hsp70-Fw </td><td>ACTGTCGACCCCGTTTAGCAGGAAATAGCC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P12 </td><td>p2 Hsp70-Rv </td><td>ACTAAGCTTATTTGCGCTCCTTACAGTTTGC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P13 </td><td>p2 Flk-1-Fw </td><td>ACTGTCGACCTGGACTCTGGGCTAGGG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P14 </td><td>p2 Flk-1-Rv </td><td>TCAAAGCTTGTTGACGTTTAGTCCAGGACAG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P15 </td><td>p2 ROSA-Fw </td><td>ACTGTCGACTAGATGAAGGAGAGCC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P16 </td><td>p2 ROSA-Rv </td><td>GATAAGCTTGGATCCCCGCAAACGCAC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P17 </td><td>p2 CarA-Fw </td><td>ACTGTCGACGTCCCATACCAGTACAATGCAAT </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P18 </td><td>p2 CarA-Rv </td><td>AGTAAGCTTGTGCTGTGATTGAATTGGCTG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P19 </td><td>IaaH-BB-Fw </td><td>gaattcgcggccgcttctagATGCGCGAAATGATTACGC </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P20 </td><td>IaaH-BB-Rv </td><td>ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P21 </td><td>IaaM-BB-Fw </td><td>gaattcgcggccgcttctagatgtttggaccggatttccc </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P22 </td><td>IaaM-BB-Rv </td><td>ctgcagcggccgctactagtattattagtcccccagcgc </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P23 </td><td>AID-Fw </td><td>cgcttctagGGAGCTGGTGCAGGCGCT </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P24 </td><td>Sp6 </td><td> </td><td> </td><td> </td><td> </td><td> <tr style="background: #cccccc;"> <td style="background: #ccffcc;">P25 </td><td>T3 </td><td> </td><td> </td><td> </td><td> </td><td> </table>

Number	Name	Sequence	Length	Tm	%GC	Comments
P1	Tir1-BB-FW	cgcttctagATGACGTACTTCCCGGAGG	19	57	58
P2	Tir1-BB-Rv	ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC	24	52	29
P3	SdMTir1-BB-Fw	AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG	65	72	43
P4	SdMTir1-BB-Rv	TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG	62	76	52
P5	Tir1 SdM v02-Fw	tgcTgtgacagaggagggac	20	60	60
P6	Tir1 SdM v02-Fw	ctagAgcagcattagtcatttgg	23	55	43
P7	pCS2+BB-Fw