Team:Evry/Freezer

From 2012.igem.org

(Difference between revisions)
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<td style="background: #ccffcc;">P24
<td style="background: #ccffcc;">P24
</td><td>Sp6
</td><td>Sp6
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</td><td>
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</td><td>ATTTAGGTGACACTATA
</td><td>
</td><td>
</td><td>
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<td style="background: #ccffcc;">P25
<td style="background: #ccffcc;">P25
</td><td>T3
</td><td>T3
-
</td><td>
+
</td><td>AATTAACCCTCACTAAAGGG
</td><td>
</td><td>
</td><td>
</td><td>

Revision as of 15:25, 1 August 2012


Freezer


Primers


Number Name Sequence Length Tm  %GC Comments
P1 Tir1-BB-FW cgcttctagATGACGTACTTCCCGGAGG 19 57 58
P2 Tir1-BB-Rv ctactagtaTTATTATAGGATTTTAACAAAATTTGGTGC 24 52 29
P3 SdMTir1-BB-Fw AACTGAACTCACTACTGTACTTCTGTCACCAAATGACTAATGCTGCTCTAGTTACTGTCGCCAAG 65 72 43
P4 SdMTir1-BB-Rv TTGGACAGCCCAAGGATACTGCAACAAGTCCCTCCTCTGTCACAGCAGAATAACCAGCTACG 62 76 52
P5 Tir1 SdM v02-Fw tgcTgtgacagaggagggac 20 60 60
P6 Tir1 SdM v02-Fw ctagAgcagcattagtcatttgg 23 55 43
P7 pCS2+BB-Fw cgctgcagGAACTATAGTGAGTCGTATTACG 23 51 39
P8 pCS2+BB-Rv tgcatGAATTCGAATCGATGGGATCC
P9 GFP-AID-BB-Fw gaattcgcggccgcttctagagATGGCCACAACCATGGTG
P10 GFP-AID-BB-Rv tactagtagcggccgctgcagTTATTAAACCTTACGTTTCTTTTTAGGGAC
P11 p2 Hsp70-Fw ACTGTCGACCCCGTTTAGCAGGAAATAGCC
P12 p2 Hsp70-Rv ACTAAGCTTATTTGCGCTCCTTACAGTTTGC
P13 p2 Flk-1-Fw ACTGTCGACCTGGACTCTGGGCTAGGG
P14 p2 Flk-1-Rv TCAAAGCTTGTTGACGTTTAGTCCAGGACAG
P15 p2 ROSA-Fw ACTGTCGACTAGATGAAGGAGAGCC
P16 p2 ROSA-Rv GATAAGCTTGGATCCCCGCAAACGCAC
P17 p2 CarA-Fw ACTGTCGACGTCCCATACCAGTACAATGCAAT
P18 p2 CarA-Rv AGTAAGCTTGTGCTGTGATTGAATTGGCTG
P19 IaaH-BB-Fw gaattcgcggccgcttctagATGCGCGAAATGATTACGC
P20 IaaH-BB-Rv ctgcagcggccgctactagtaTTATTAGCCTTTTAACACTTTTTCG
P21 IaaM-BB-Fw gaattcgcggccgcttctagatgtttggaccggatttccc
P22 IaaM-BB-Rv ctgcagcggccgctactagtattattagtcccccagcgc
P23 AID-Fw cgcttctagGGAGCTGGTGCAGGCGCT
P24 Sp6 ATTTAGGTGACACTATA
P25 T3 AATTAACCCTCACTAAAGGG