Team:Evry/Data

From 2012.igem.org

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<h2>Reporters and promotors</h2>
<h2>Reporters and promotors</h2>
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<h3>Summery<h3>
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In our Project we planned to screen a series of different reporters  along with different tissue specific promotors. This was done to validate cross-talk between the emitter-receiver system and and screen  various reporters to elucidate the efficiency of different reporters in Tadpole manipulation.
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<p> <ul>
<p> <ul>
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812030">K812030:</a>Citrine reporter with a Kozak sequence for eukaryotic expression
<li><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K812030">K812030:</a>Citrine reporter with a Kozak sequence for eukaryotic expression
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<h3>Characterization<h3> the information on characterized part can be found herein: <ul>
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<h2>Characterization<h2> the information on characterized part can be found herein: <ul>
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<li><a href="http://2012.igem.org/Team:Evry/FrenchFrog">FrenchFrog:</a>
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<li><a href="http://2012.igem.org/Team:Evry/FrenchFrog">FrenchFrog:</a> ,/ul>

Revision as of 00:18, 25 September 2012

Data page: Summary of what we have done this year (so far!)



New frog plasmids

Fig 1: Eucaryotic expression plasmid having both transcriptional and translational reagulational elements.

  • K812000: PCS2+ plasmid backbone for eukaryotic expression system containing regulational elements crucial for the efficacy of such system

The auxin system

The auxin receiver system

Fig 2: schemetic representation of the Auxin-receiver system

  • K812010:GFP fusion with the ubuquitinase E3 OsTirI recoginition domain which a main part of the degron system
  • K812012:OsTirI Ubiquitinase E3 for AID tagged protein degradation in the presence of auxin
  • K812013:

    The Auxin reciever system golden gate-assembled containing GFP-AID OsTirI polysistronic system for auxin detection in tadpole

The auxin emission system

Fig 3: Schematic representation of the Auxin two-step production cassette.

  • K812020:IAAH enzyme that catalyzes the second and last step towards auxin production
  • K812021:IAAM enzyme that catalyses the transformation of Tryptophan to Inodle-3-acetamide
  • K812014:Auxin production cassette containg both enzymes above which is monocystronic

Reporters and promotors

Summery

In our Project we planned to screen a series of different reporters along with different tissue specific promotors. This was done to validate cross-talk between the emitter-receiver system and and screen various reporters to elucidate the efficiency of different reporters in Tadpole manipulation.

  • K812030:Citrine reporter with a Kozak sequence for eukaryotic expression
  • K812031:sfGFP with kozak sequence for eukaryotic expression
  • K812032:mCFP with kozak sequence for eukaryotic expression
  • K812200:Biobricked pSC2+ plasmid with Elastase promoter for frogs and chicken
  • K812300:Biobricked pSC2+ plasmid with HSP70 promoter for frogs and chicken
  • K812233:sfGFP in pCS2+ downstream to elastase promoter
  • K812331:sfGFP in pCS2+ downstream to HSP70 promoter

Characterization

the information on characterized part can be found herein:
  • FrenchFrog: ,/ul>

    Goldenbricks

    Summary

    Fig 4: Summary of the GoldenBrick procedure

    The GoldenBrick is a new assembly method for the partsregistry. If you want to know more, see this page.

    Our favourites parts

    • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
    • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control

    The other parts we have created

    • K812050: A GoldenBricked version of pSB1C3 with J04450 as negative cloning control
    • K812051: A GoldenBricked version of pSB1K3 with J04450 as negative cloning control
    • K812053: A GoldenBricked version of the strong RBS B0034
    • K812054: A GoldenBricked version of the RFP E1010
    • K812055: A GoldenBricked version of the terminator B0015
    • K812056: A GoldenBricked version of the pLac R0010 promoter
    • K812057: A GoldenBricked of an sfGFP protein
    • K812058: A GoldenBricked of medium strenght RBS J61107
    • K812059: A GoldenBricked of week RBS J61117

    say we are testing them