Part assembly
We planned to clone several parts we used into pSB1C3, which is the standard backbone plasmid in which parts are shipped to the Registry[1]. Four parts were successfully inserted based on colony PCR results followed by successful sequencing.
LovTAP-VP16: BBa_K838000 [2]
LovTAP readout (RO): BBa_K838001 [3]
Melanopsin: BBa_K838002 [4]
NFAT response element: BBa_K838003 [5]