Team:EPF-Lausanne/Results

From 2012.igem.org

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'''LovTAP-VP16:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000 BBa_K838000]
'''LovTAP-VP16:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000 BBa_K838000]
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[[File:Team-EPF-Lausanne LovTAPbb.JPG|600px]]
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Successfully cloned [https://2012.igem.org/Team:EPF-Lausanne/Notebook/4_September_2012 4th of September].
'''LovTAP readout (RO):''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001 BBa_K838001]
'''LovTAP readout (RO):''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001 BBa_K838001]
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[[File:Team-EPF-Lausanne pSB1C3-RO.JPG|600px]]
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Successfully cloned on [https://2012.igem.org/Team:EPF-Lausanne/Notebook/18_September_2012 18th of September]
'''Melanopsin:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002 BBa_K838002]
'''Melanopsin:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002 BBa_K838002]
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Successfully cloned on [https://2012.igem.org/Team:EPF-Lausanne/Notebook/12_September_2012 12th of September]
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[[File:Team-EPF-Lausanne melanopsinbb.JPG|600px]]
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'''NFAT response element:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838003 BBa_K838003]
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[[File:Team-EPF-Lausanne pSB1C3-NFAT.JPG|600px]]
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Revision as of 09:33, 26 September 2012

Contents

Transfections

Show the 30-50% efficiency.

Flowcytometry

Detecting LovTAP

Talk about shifting peak (and why absent in PBS one: dsRed is degraded by formaldehyde)

Characterizing LovTAP

Non-leakage DsRed, where art thou?

Part assembly

We planned to clone several parts we used into pSB1C3, which is the standard backbone plasmid in which parts are shipped to the [http://partsregistry.org/Main_Page Registry]. Four parts were successfully inserted based on colony PCR results followed by successful sequencing.

LovTAP-VP16: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000 BBa_K838000]

Successfully cloned 4th of September.

LovTAP readout (RO): [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001 BBa_K838001]

Successfully cloned on 18th of September

Melanopsin: [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002 BBa_K838002] Successfully cloned on 12th of September


Wiki Links

Important pages

Pages

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