Team:EPF-Lausanne/Results

From 2012.igem.org

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'''LovTAP-VP16:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000 BBa_K838000]
'''LovTAP-VP16:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838000 BBa_K838000]
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[[File:Team-EPF-Lausanne LovTAPbb.JPG|600px]]
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Successfully cloned [https://2012.igem.org/Team:EPF-Lausanne/Notebook/4_September_2012 4th of September].
'''LovTAP readout (RO):''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001 BBa_K838001]
'''LovTAP readout (RO):''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838001 BBa_K838001]
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[[File:Team-EPF-Lausanne pSB1C3-RO.JPG|600px]]
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Successfully cloned on [https://2012.igem.org/Team:EPF-Lausanne/Notebook/18_September_2012 18th of September]
'''Melanopsin:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002 BBa_K838002]
'''Melanopsin:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838002 BBa_K838002]
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Successfully cloned on [https://2012.igem.org/Team:EPF-Lausanne/Notebook/12_September_2012 12th of September]
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[[File:Team-EPF-Lausanne melanopsinbb.JPG|600px]]
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'''NFAT response element:''' [http://partsregistry.org/wiki/index.php?title=Part:BBa_K838003 BBa_K838003]
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[[File:Team-EPF-Lausanne pSB1C3-NFAT.JPG|600px]]
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Revision as of 09:33, 26 September 2012

Contents

Transfections

Show the 30-50% efficiency.

Flowcytometry

Detecting LovTAP

Talk about shifting peak (and why absent in PBS one: dsRed is degraded by formaldehyde)

Characterizing LovTAP

Non-leakage DsRed, where art thou?

Part assembly

We planned to clone several parts we used into pSB1C3, which is the standard backbone plasmid in which parts are shipped to the Registry. Four parts were successfully inserted based on colony PCR results followed by successful sequencing.

LovTAP-VP16: BBa_K838000

Successfully cloned 4th of September.

LovTAP readout (RO): BBa_K838001

Successfully cloned on 18th of September

Melanopsin: BBa_K838002 Successfully cloned on 12th of September


Wiki Links

Important pages

Pages

Retrieved from "http://2012.igem.org/Team:EPF-Lausanne/Results"